We narrowed to 15,731 results for: LEA

1. pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

   Plasmid

   #52539

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMT-Map7-1333-end

   Plasmid

   #46070

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-1333-end (Map7 Mouse)
   

   Expression

   Bacterial

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. ALFY-FYVE 1G

   Plasmid

   #197045

   Purpose

   Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

   Insert

   ALFY (WDFY3 Human)
   

   Tags

   6xHis-GST-TEV

   Expression

   Bacterial

   Available Since

   Dec. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-FKBP-mTagBFP2-SOScat

   Plasmid

   #178866

   Purpose

   Mammalian expression of SOS1(Rem-Cdc25) fused to FKBP-mTagBFP2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   FKBP-mTagBFP2-SOS(Rem-Cdc25) (SOS1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIG-Flag-RIPK1-wt

   Plasmid

   #102677

   Purpose

   retroviral expression plasmid for RIPK1-wt

   Depositor

   Hasam Habelhah

   Article

   Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and IRES-GFP

   Expression

   Mammalian

   Promoter

   unknown

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-mCherry.3xFLAG-WPRE

   Plasmid

   #127861

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   mCherry
   

   Use

   AAV

   Tags

   3xFLAG

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInsert-PhoCl-RER

   Plasmid

   #219676

   Purpose

   Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

   Depositor

   Helge Ewers

   Article

   Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

   Insert

   CD4mScarlet-PhoCl-RER
   

   Tags

   mScarlet and PhoCl

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 14, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pTY153 EGFP-T2A-ORF6 SARS-CoV-2

   Plasmid

   #204975

   Purpose

   Co-express EGFP and SARS-CoV-2 ORF6

   Depositor

   Tim Mitchison

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   Open Reading Frame 6 (ORF6 SARS-CoV-2)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y

    Plasmid

    #148087

    Purpose

    Insect Expression of DmPAN3-W707AG711E-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
    

    Expression

    Insect

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV E2F1 1-284

    Plasmid

    #24216

    Purpose

    Mammalian expression of human E2F1 (aa 1-284) with HA tag

    Depositor

    Kristian Helin

    Article

    Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

    Insert

    E2F1 1-284 (E2F1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pTY152 EGFP-T2A-ORF6 SARS-CoV-1

    Plasmid

    #204974

    Purpose

    Co-express EGFP and SARS-CoV-1 ORF6

    Depositor

    Tim Mitchison

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    Open Reading Frame 6 (ORF6 SARS-CoV-1)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+MT-hToca-1-MGD

    Plasmid

    #33033

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    MGD = amino acid substitutions M383I, G384S and D…

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

14. BirA*-HA-NES

    Plasmid

    #214473

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and NES (Nuclear Exclusion Sequence)

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HS-aPKC-CAAX-GFP

    Plasmid

    #105946

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    aPKC (Prkcz Rat)
    

    Tags

    CAAX and GFP

    Expression

    Bacterial

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC267

    Plasmid

    #118887

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease; HALO tag

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLS.416

    Plasmid

    #187207

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2)
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQE80l B-CN-B

    Plasmid

    #196477

    Purpose

    Protein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    B-CN-B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1a-V5-Sec61B

    Plasmid

    #120244

    Purpose

    Expresses V5-tagged Sec61B in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Sec61B (Sec61b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only