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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB170

Plasmid

#218591

Purpose

Express engineered TFs Adr1c(S230A), Pip2c, and Oaf1c

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Adr1

Expression

Yeast

Mutation

Adr1(S230A), Pip2(1-168 + 2,497-2,988), Oaf1(1-30…

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1340

Plasmid

#218593

Purpose

Express a red fluorescent protein to the peroxisome membrane via tethering to a truncated Pex22. Also overexpresses Pex11

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex22

Expression

Yeast

Mutation

Pex22(1-36)

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSKT001

Plasmid

#218594

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1357

Plasmid

#218595

Purpose

Express Pex11 and the degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Pex11

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pKS1461

Plasmid

#218587

Purpose

Express peroxisomal Erg12p, Erg8p, and Mvd1p to convert mevalonate to IPP

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

Erg12

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTE5417_p3WJdB_promoter dropout vector

Plasmid

#221191

Purpose

Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Use

Synthetic Biology

Promoter

Promoter Placeholder/Dropout Vector

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-196a.M3

Plasmid

#220467

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a

Plasmid

#220470

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-7

Plasmid

#220464

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

fuGFP

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-7.M4

Plasmid

#220465

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_VPS26C

Plasmid

#209690

Purpose

Expresses MAC-tagged VPS26C in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS26C (VPS26C Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_VPS35L

Plasmid

#209692

Purpose

Expresses MAC-tagged VPS35L in mammalian cells

Depositor

Markku Varjosalo

Article

Laulumaa et al Nat Struct Mol Biol. 2024 Jun;31(6):925-

Insert

VPS35L (VPS35L Human)

Tags

MAC-tag

Expression

Mammalian

Mutation

NC_000016.9:g.19710824G>A

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTvGPI(EP)-blast-mNG

Plasmid

#221051

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTvGPI(VSG)-blast-mNG

Plasmid

#221050

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_A_miR-196a

Plasmid

#220466

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version A)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Superfolder GFP

Tags

3xFLAG

Expression

Mammalian

Promoter

Minimal CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-H2B-MCS

Plasmid

#184041

Purpose

Cloning of GFP-H2B fusion proteins

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Type

Empty backbone

Tags

GFP and H2B

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-175

Plasmid

#99668

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Tags

truncated RTA activation domain (125-175)

Expression

Mammalian

Mutation

dead Cas9

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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