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Hp MTAN

Plasmid

#69802

Purpose

Expression of H. pylori J99 methylthioadenosine nucleosidase

Depositor

Vern Schramm

Publication

Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

Insert

Methylthioadenosine nucleosidase

Tags

Histidine tag

Expression

Bacterial

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pShuttle

Plasmid

#67964

Purpose

Mobilizible plasmid with EGFP. OriT from the F plasmid.

Depositor

Joshua Leonard

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OriT

Use

Synthetic Biology

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0661

Plasmid

#133476

Purpose

Expression in fission yeast from promoter pact1, integrated at ade6 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-SNAP-CLIP

Plasmid

#127180

Purpose

Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

Depositor

Kai Johnsson

Publication

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-CLIP

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Publication

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Expression

Mammalian

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-Renilla

Plasmid

#79496

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Renilla Luciferase

Use

Gateway Cloning

Promoter

no Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY (ABE8e_V28C_nCas9_SpRY)

Plasmid

#251544

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Publication

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY

Use

CRISPR

Expression

Mammalian

Mutation

ABEx1 V28C

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pGL4.R_8xGLIBS_minPro_Luc2p_Ren

Plasmid

#249281

Purpose

Dual-luciferase GLI reporter plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression.

Depositor

Ramiro Iglesias-Bartolome

Publication

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

Luciferase

Use

Luciferase

Expression

Mammalian

Available since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-20nmEML-mRFP

Plasmid

#231181

Purpose

Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 20 nm from a lentiviral vector

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Lentiviral

Promoter

CMV

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Publication

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAV0753

Plasmid

#133487

Purpose

Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-T7-SdB-T

Plasmid

#87307

Purpose

Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Stabilized dimeric Broccoli aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPd4E-ISH

Plasmid

#61700

Purpose

Plum eIF4E ihp-RNA construct

Depositor

Lining Tian

Publication

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF4E

Use

RNAi

Promoter

35S

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR-RNF40i1

Plasmid

#59594

Purpose

Expression of shRNA against human RNF40

Depositor

Miguel Ramalho-Santos

Publication

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

RNF40 (RNF40 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pcDKIER

Plasmid

#37093

Depositor

David Wang

Insert

KI polyomavirus early region (KPV_gp4, KPV_gp5 KI polyomavirus)

Expression

Mammalian

Promoter

CMV

Available since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV.SP146.gp91.GP91.cHS4

Plasmid

#30480

Depositor

Didier Trono

Publication

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/GP91/cHS4

Use

Lentiviral

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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V17V

Plasmid

#29424

Depositor

Steven Vogel

Publication

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus-17-Venus

Use

Anisotropy and brightness standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
ITGB2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176983

Purpose

Mammalian expression vector encoding ITGB2 and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ITGB2 (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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