We narrowed to 16,996 results for: bon

1. pInducer-myc-ERK1-S170D

   Plasmid

   #213592

   Purpose

   Doxycycline inducible expression of myc-tagged ERK1-S170D cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   S170D

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pInducer-myc-ERK1-R84S/D338N

   Plasmid

   #213594

   Purpose

   Doxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   R84S, D338N

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1+FLAG-KrascomQ61R

   Plasmid

   #206845

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Promoter

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. TR01F/hFGFR3-G380R-V5

   Plasmid

   #214908

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR3-G380R variant that is associated with Achondroplasia

   Depositor

   Pavel Krejčí

   Article

   Kimura et al Sci Transl Med. 2021 May 5;13(592):eaba4

   Insert

   FGFR3 (FGFR3 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   G380R substitution

   Promoter

   truncated CMV promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

   Plasmid

   #169163

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCJ200

   Plasmid

   #162673

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C and a sequence Y75-F104 to introduce a 6th…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCJ210

   Plasmid

   #162683

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   S136C, G489C, S530C, and a sequence Y75-F104 to i…

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

   Plasmid

   #120404

   Purpose

   enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine

   Depositor

   Inaam Nakchbandi

   Article

   Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

   Insert

   Fibronectin (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Containing complete variable region with a mutati…

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Fibronectin-human-mutated V2 O-Glycosylation site

    Plasmid

    #120405

    Purpose

    enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine

    Depositor

    Inaam Nakchbandi

    Article

    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.

    Insert

    Fibronectin (FN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains complete variable region with a mutation…

    Promoter

    CMV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLgw EcoDam-V5-ELK4

    Plasmid

    #98606

    Purpose

    Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_ARSI_WT

    Plasmid

    #81898

    Purpose

    Gateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ARSI (ARSI Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_GPR141_WT

    Plasmid

    #81876

    Purpose

    Gateway Donor vector containing GPR141 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    GPR141 (GPR141 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_CNBD1_WT

    Plasmid

    #81877

    Purpose

    Gateway Donor vector containing CNBD1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CNBD1 (CNBD1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_HERPUD2_WT

    Plasmid

    #81880

    Purpose

    Gateway Donor vector containing HERPUD2 ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    HERPUD2 (HERPUD2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    A108T

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_IKZF5_WT

    Plasmid

    #81855

    Purpose

    Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IKZF5 (IKZF5 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    M204V; p.415_418delQHNQ; p.419_420-insITNID

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_C20orf72_WT

    Plasmid

    #81806

    Purpose

    Gateway Donor vector containing C20ORF72 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    C20orf72 (MGME1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_GZMA_WT

    Plasmid

    #81792

    Purpose

    Gateway Donor vector containing GZMA , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    GZMA (GZMA Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Y5N

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_HRH4_WT

    Plasmid

    #81786

    Purpose

    Gateway Donor vector containing HRH4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    HRH4 (HRH4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223_KRT26_WT

    Plasmid

    #81763

    Purpose

    Gateway Donor vector containing KRT26 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    KRT26 (KRT26 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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