We narrowed to 6,927 results for: tac

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pGREAT12

Plasmid

#170900

Purpose

GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtACT2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

p35SoTMV

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGREAT13

Plasmid

#170901

Purpose

GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtACT2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pAtUBQ10

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGREAT14

Plasmid

#170902

Purpose

GoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtACT2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pG10-90

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-PRGRH-Nluc

Plasmid

#158608

Purpose

Plasmid expressing PRG(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pKB235

Plasmid

#170024

Purpose

Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL20 (CCL20 Human, Synthetic, Escherichia coli)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-p115RH-Nluc

Plasmid

#158607

Purpose

Plasmid expressing p115(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-U6a-U6c-mfn2 guide#2

Plasmid

#121994

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen N-term (WS14)

Plasmid

#126875

Purpose

NeonGreen-tag attached to the N-terminus of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Tags

NeonGreen

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pKD286

Plasmid

#125074

Purpose

Expression of SO_4622 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4622 (SO_4622 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKD276

Plasmid

#125069

Purpose

Expression of SO_0621 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_0621 (SO_0621 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ4

Plasmid

#126407

Purpose

Expression of Smoothened NQ4 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Mutation

Smo glycosylation site NQ4

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened NQ3

Plasmid

#126406

Purpose

Expression of Smoothened NQ3 in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Expression

Mammalian

Mutation

Smo glycosylation site NQ3

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL602 CG

Plasmid

#126953

Purpose

To target a protospacer with CG at the cut site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CG at cut site

Expression

Mammalian

Promoter

human U6

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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gh148

Plasmid

#106818

Purpose

expression of gRNA targeting KDELC2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

KDELC2 (POGLUT3 Human)

Expression

Mammalian

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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gh96

Plasmid

#106756

Purpose

expression of gRNA targeting POFUT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

POFUT1 (POFUT1 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh47

Plasmid

#106718

Purpose

expression of gRNA targeting ST6GALNAC6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST6GALNAC6 (ST6GALNAC6 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK519

Plasmid

#121476

Purpose

CEN TRP1 CNA1ΔCBD

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNA1deltaCBD

Use

Yeast cen vector

Mutation

Truncated after amino acid 453

Promoter

Endogenous genomic promoter region

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 CR3m shRNA

Plasmid

#120795

Purpose

Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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gamma2 UTR3m shRNA

Plasmid

#120797

Purpose

Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

Depositor

Angel de Blas

Article

Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

Insert

GABRG2 shRNA (Gabrg2 Rat)

Use

RNAi

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-SUV39h1 ts3

Plasmid

#115882

Purpose

SUV39h1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SUV39h1 shRNA (SUV39H1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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