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pBBK64 pHomL-RPL18Bp-PEX25-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179048

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Neon(gfp), a marker for the peroxisome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PEX25-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK63 pHomL-RPL18Bp-PDR16-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179047

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PDR16-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK62 pHomL-RPL18Bp-ERG6-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179046

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ERG6-Link-Neon(gfp), a marker for the lipid droplet

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ERG6-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK61 pHomL-RPL18Bp-Neon(gfp)-Link-ATG8-SSA1t-HomR (AmpR)

Plasmid

#179045

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-ATG8, a marker for the autophagosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-ATG8

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK60 pHomL-RPL18Bp-PRC1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179044

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PRC1-Link-Neon(gfp), a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PRC1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK59 pHomL-RPL18Bp-Neon(gfp)-Link-VAM3-SSA1t-HomR (AmpR)

Plasmid

#179043

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VAM3, a marker for the vacuole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VAM3

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK58 pHomL-RPL18Bp-Neon(gfp)-Link-YPT7-SSA1t-HomR (AmpR)

Plasmid

#179042

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-YPT7, a marker for the vacuole, late endosome

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-YPT7

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK57 pHomL-RPL18Bp-SNF7-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179041

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SNF7-Link-Neon(gfp), a marker for the endosome, class E compartment

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SNF7-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK56 pHomL-RPL18Bp-Neon(gfp)-Link-VPS21-SSA1t-HomR (AmpR)

Plasmid

#179040

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-VPS21, a marker for the endosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-VPS21

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK55 pHomL-RPL18Bp-PIL1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179039

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of PIL1-Link-Neon(gfp), a marker for the eisosomes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-PIL1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK53 pHomL-RPL18Bp-Neon(gfp)-Link-SFT2-SSA1t-HomR (AmpR)

Plasmid

#179037

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SFT2, a marker for the late Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SFT2

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK52 pHomL-RPL18Bp-Neon(gfp)-Link-SED5-SSA1t-HomR (AmpR)

Plasmid

#179036

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-SED5, a marker for the early Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-SED5

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK50 pHomL-RPL18Bp-ARC35-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179034

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Neon(gfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Neon(gfp)

Expression

Yeast

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK48 pHomL-RPL18Bp-SPC42-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179032

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Neon(gfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Neon(gfp)

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK47 pHomL-RPL18Bp-SRM1-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179031

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SRM1-Link-Neon(gfp), a marker for the nucleus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SRM1-Link-Neon(gfp)

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK46 pHomL-RPL18Bp-RPC19-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179030

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of RPC19-Link-Neon(gfp), a marker for the nucleolus

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-RPC19-Link-Neon(gfp)

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtU6-26:AsCas12a_DR (GB1442)

Plasmid

#160574

Purpose

AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtU6-26:AsCas12a_DR

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pOCC176

Plasmid

#118891

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette

Tags

HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

Expression

Insect

Promoter

polH

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET-30a(+)-N-SH2

Plasmid

#111274

Purpose

express murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)

Tags

HHHHHHENLYFQG

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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SP65U1-135a-1

Plasmid

#105819

Purpose

plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid

Depositor

Cecilia Mannironi

Article

Mannironi et al PLoS One. 2013 Sep 4;8(9):e73385. doi: 1

Insert

MIRNA135A1 (Mir135a-1 Mouse)

Expression

Mammalian

Promoter

U1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMGF172

Plasmid

#96998

Purpose

ish1 mCherry insertion cassette with ura4+ gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + ura4(…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437) L151D

Plasmid

#87736

Purpose

Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Leu 151 to Asp

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

Plasmid

#83462

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, W4107A

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

Plasmid

#83460

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

Plasmid

#83463

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4169

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

Plasmid

#83459

Purpose

Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4107-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

Plasmid

#83457

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS L4137P-GST

Plasmid

#83455

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at L4137 and a GST-tag (848)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, L4137P

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS R4150C-GST

Plasmid

#83454

Purpose

Human PC1-CTp30 expression cassette with a patient mutation at R4150 and a GST-tag (850)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Mammalian

Mutation

Amino-acids 4107-4303, R4150C

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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