We narrowed to 12,952 results for: sequence

1. pCA-ATG-intron-tdT3Myc

   Plasmid

   #36883

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tdTomato-3Myc
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   beta-globin intron containing a loxP site inserte…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. miR-144 Trap

   Plasmid

   #60934

   Purpose

   to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   miR-144 Trap
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   bidirectional phosphoglycerate kinase promoter (b…

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pLyGo-Ec-6

   Plasmid

   #163132

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPT-ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49229

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH353-SUP45wt

   Plasmid

   #29371

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Wild type gene including genomic sequence 1432 bp…

   Promoter

   Available Since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

   Plasmid

   #140200

   Purpose

   Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   dLbCas12a crRNA scaffold

   Expression

   Mutation

   Promoter

   PgpdA

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a-T2-NLuc

   Plasmid

   #135938

   Purpose

   Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   T2 mitochondrial localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. actc1b-mKate2-GPI-pA

   Plasmid

   #109490

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-GPI-pA
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLyGo-Kp-2

   Plasmid

   #163144

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-Cx35-Halo-C-in

    Plasmid

    #71511

    Purpose

    Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

    Depositor

    John O'Brien

    Article

    Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

    Insert

    Connexin 35
    

    Use

    Tags

    HaloTag (internal to sequence)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTK4b_004

    Plasmid

    #123843

    Purpose

    Encodes spacer sequence as a Type 4b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Spacer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HOXD12 (murine) pRSETa

    Plasmid

    #8554

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXD12 (Hoxd12 Mouse)
    

    Use

    Tags

    HIS and XPress

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pAc5.1B-EGFP-DmLSm4_A

    Plasmid

    #145883

    Purpose

    Insect Expression of DmLSm4

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

    Insert

    DmLSm4 (LSm-4 Fly)
    

    Use

    Tags

    V5-6xHis

    Expression

    Insect

    Mutation

    two silent mutations compared to the sequence of …

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49218

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ABKAR-Kras

    Plasmid

    #84637

    Purpose

    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, C…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GG-EBNA-TdT-guide1-PGK-Puro

    Plasmid

    #102903

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    TdT-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK4a_005

    Plasmid

    #123819

    Purpose

    Encodes Mitochondria Targeting Sequence as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    MTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTK4a_007

    Plasmid

    #123821

    Purpose

    Encodes Peroxisome Targeting Sequence as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PTS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MTK4a_002

    Plasmid

    #123816

    Purpose

    Encodes Nuclear Export sequence as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK3a_002

    Plasmid

    #123755

    Purpose

    Encodes Nuclear Export sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MTK3a_003

    Plasmid

    #123756

    Purpose

    Encodes Nuclear import sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MTK3a_004

    Plasmid

    #123757

    Purpose

    Encodes Golgi Targeting Sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    GTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MTK3a_005

    Plasmid

    #123758

    Purpose

    Encodes Mitochondria Targeting Sequence as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    MTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTH371-SUP45-N58A

    Plasmid

    #29378

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N58A mutant gene including genomic sequence 1432 …

    Promoter

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

25. D92_Ld_360170

    Plasmid

    #135905

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    hypothetical protein, conserved
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pWPT-/UAA-mEGFP-IRES-mCherry

    Plasmid

    #49234

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28-At_PRORP3-His

    Plasmid

    #67871

    Purpose

    bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP3 (PRORP3 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRPB1-CTD14

    Plasmid

    #112822

    Purpose

    pSMF2 with 14 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJBL3664

    Plasmid

    #162240

    Purpose

    Transcribes E. coli SRP RNA sequence for SHAPE-Seq

    Depositor

    Julius Lucks

    Article

    Yu et al Mol Cell. 2021 Feb 18;81(4):870-883.e10.

    Inserts

    E. coli SRP RNA
    HDV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVC-Ds-DrU6a:sgRNA-Ds

    Plasmid

    #119069

    Purpose

    To clone sgRNA spacer sequence for microinjections in zebrafish

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge