We narrowed to 27,126 results for: STI

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-His-VHL-Flag

Plasmid

#29497

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Tags

Flag and His

Expression

Insect

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
CpcB-Solanum-lycopersicumPHLS_CpcA

Plasmid

#74003

Purpose

Expresses the Solanum lycopersicum PHLS as a fusion with the CpcB protein plus the NPPS and CmR cassete in Synechocystis

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

Inserts

PHLS and CmR
Neryl diphosphate synthase from Solanum lycopersicum

Promoter

cpc

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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MTK0_043

Plasmid

#123943

Purpose

Encodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - A1 Insulator - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

Plasmid

#100979

Purpose

Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

Depositor

Heinrich Leonhardt

Article

Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

Insert

c-Myc NLS-FKBP-Venus N-T2A-mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSW177 - pCASP1

Plasmid

#115986

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Alexis Maizel

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pCASP1

Use

Unspecified

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEM-WingGFP-tan

Plasmid

#83473

Purpose

EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

Depositor

Patricia Wittkopp

Article

Lamb et al Fly (Austin). 2016 Aug 5:1-12.

Insert

WingGFP-tan

Use

CRISPR

Expression

Bacterial and Insect

Promoter

Hsp70 and enhancer from D. melanogaster yellow ge…

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pJAF213

Plasmid

#52403

Purpose

Expresses Flag-tagged mouse Arf2

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf2 (Arf2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
VPS15

Plasmid

#171418

Purpose

For the purifcation of human PI3KC3 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

VPS15 (PIK3R4 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJH3043

Plasmid

#175284

Purpose

Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of isoform a of DAF-16 fused to GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

daf-16a mini-gene (daf-16 Nematode)

Expression

Worm

Promoter

Pdpy-30

Available Since

Nov. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJH199_pE12_hyCal2-mCherry

Plasmid

#160412

Purpose

Gateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to mCherry

Depositor

Daniel Colón-Ramos

Article

Hawk et al Nat Commun. 2021 Aug 9;12(1):4795. doi:

Insert

pDONR221 HyCal2::mCherry

Use

Other

Tags

mCherry

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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XE242 Mouse CK2alpha CS2p+

Plasmid

#16730

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

mouse Casein Kinase II alpha-pCS2P+ (Csnk2a1 Mouse)

Use

Xenopus expression

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pCFJ66

Plasmid

#24981

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119 Punc-122::GFP

Use

Multisite gateway

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pGW-FL-V5

Plasmid

#78858

Purpose

DULIP Prey Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-RL-PA

Plasmid

#78856

Purpose

DULIP Bait Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Renilla luciferase, Protein A

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K19R/K49R

Plasmid

#44751

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R/K49R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pMD19T-psba1-Ppsba2-dCas9-SpR

Plasmid

#73220

Purpose

Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

Depositor

Paul Hudson

Article

Yao et al ACS Synth Biol. 2015 Dec 28.

Insert

dCas9 from S. pyogenes

Tags

c-myc

Expression

Bacterial

Mutation

Silent mutation at bp 1341 A->C to remove an E…

Promoter

PpsbA2

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pPJ173(JPUB_006777)

Plasmid

#83834

Purpose

Carries NEW operon 10 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3336
Kuste3339
Kuste3341

Promoter

op10_3339_for, op10_3341_for, and op10_BsaI_3336_…

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPJ174(JPUB_006779)

Plasmid

#83827

Purpose

Carries operon 1 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3603
kuste3605
kuste3606

Use

Pfab217 (p15a ori, kanr)

Promoter

op1_3603_for, op1_3605_for, and op1_3606_for

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPU-Empty-miR30-L1221

Plasmid

#132940

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pPU-huTRIMmiR30-L1221

Plasmid

#132937

Purpose

All-in-one huTRIM5 rescue-control shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HA-Amida

Plasmid

#19794

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

Amida (TFPT Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 24, 2008

Availability

Academic Institutions and Nonprofits only
pJH2947

Plasmid

#132362

Purpose

Prgef-1 INS-2::GFP unc-54 3' UTR C.elegans pan-neural expression of INS-2::GFP

Depositor

Mei Zhen

Article

Hung et al Development. 2014 Apr;141(8):1767-79. do

Insert

INS-2 (ins-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Prgef-1

Available Since

Oct. 7, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHMWGFPR

Plasmid

#64396

Purpose

High molecular weight glutenin gene promoter driving GFP expression

Depositor

Claudia Vickers

Article

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

High molecular weight glutenin gene promoter

Tags

GFP

Expression

Plant

Promoter

High molecular weight glutenin gene promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
BacPAK Flag-NFRKB

Plasmid

#19791

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

Flag and His

Expression

Insect

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
p667-UBC-AAK1-V5-TurboID_IDG-K

Plasmid

#135256

Purpose

Gateway destination clone of AAK1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AAK1-V5-TurboID (AAK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Promoter

Ubiquitin

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_002

Plasmid

#123960

Purpose

Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB Destination - 2xHS4 - HygroR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRFP shRNA-resistant TrkB WT

Plasmid

#72714

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
prLCA-Citrine

Plasmid

#40271

Depositor

Martin Offterdinger

Article

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

Clta (Clta Rat)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3_4xUAS_CP-candidate_luc+

Plasmid

#125154

Purpose

STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Luciferase

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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