We narrowed to 9,370 results for: BLI

1. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

   Plasmid

   #195623

   Purpose

   Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct

   Depositor

   Tonia Rex

   Insert

   dCasphi
   

   Tags

   C-Myc (NLS), HA tag, and ZIM3

   Expression

   Mammalian

   Mutation

   P749A and P753A

   Promoter

   EF1a (Mlu1/EcoR1)

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVLINKv1-pCALM1-Cre-3' NUP155

   Plasmid

   #236645

   Purpose

   3' AAVLINK plasmid for NUP155 expression

   Depositor

   Zhonghua Lu

   Insert

   NUP155 (NUP155 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Promoter

   pCALM1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVLINKv1-pCALM1-Cre-3' PRR12

   Plasmid

   #236677

   Purpose

   3' AAVLINK plasmid for PRR12 expression

   Depositor

   Zhonghua Lu

   Insert

   PRR12 (PRR12 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Promoter

   pCALM1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVLINKv1-pCALM1-Cre-3' NINL

   Plasmid

   #236691

   Purpose

   3' AAVLINK plasmid for NINL expression

   Depositor

   Zhonghua Lu

   Insert

   NINL (NINL Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Promoter

   pCALM1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVLINKv1-pCALM1-Cre-3' UBN2

   Plasmid

   #236679

   Purpose

   3' AAVLINK plasmid for UBN2 expression

   Depositor

   Zhonghua Lu

   Insert

   UBN2 (UBN2 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Promoter

   pCALM1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVLINKv1-pCALM1-Cre-3' PHRF1

   Plasmid

   #236707

   Purpose

   3' AAVLINK plasmid for PHRF1 expression

   Depositor

   Zhonghua Lu

   Insert

   PHRF1 (PHRF1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Promoter

   pCALM1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAVLINKv1-pCALM1-Cre-3' PREX1

   Plasmid

   #236709

   Purpose

   3' AAVLINK plasmid for PREX1 expression

   Depositor

   Zhonghua Lu

   Insert

   PREX1 (PREX1 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG

   Promoter

   pCALM1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSR23: pET-SUMO-USP21 (1-565)

   Plasmid

   #240189

   Purpose

   Bacterial expression for USP21 (1-565)

   Depositor

   Cynthia Wolberger

   Insert

   USP21 (USP21 Human)
   

   Tags

   6x-His-SUMO

   Expression

   Bacterial

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQdCas12a.sggfp(A)

   Plasmid

   #236038

   Purpose

   The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQdCas12a.sggfp(B)

    Plasmid

    #236040

    Purpose

    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_852

    Plasmid

    #185527

    Purpose

    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Inserts

    First sgRNA cassette
    Second sgRNA cassette
    

    Use

    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt

    Expression

    Mammalian

    Promoter

    Human U6 and Mouse U6

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-tdTomato

    Plasmid

    #232631

    Purpose

    catalytically inactive dCas9 for localization; labeled with tdTomato

    Depositor

    Karsten Rippe

    Insert

    dCas9-tdTomato
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV (TATA box removed)

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1996_pCCLsin.PPT.hPGK.EGFP-mCEP83.PRE

    Plasmid

    #231805

    Purpose

    Label the mother centrosome-specific distal appendage protein CEP83 by electroporating an mRNA encoding it as well as an eGFP fusion protein.

    Depositor

    Annette Oxenius

    Insert

    eGFP-mCEP83
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti puro HA-STX3

    Plasmid

    #227590

    Purpose

    Lentiviral plasmid expressing human STX3 protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    STX3 (STX3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-MM002-ZFP143-C-terminal-sgRNA

    Plasmid

    #212706

    Purpose

    Plasmid for transient expression of pspCas9(BB)-2A-Puro nuclease and sgRNA targetting the C terminal of Zfp143 locus

    Depositor

    Elzo de Wit

    Insert

    ZFP143 C-terminal sgRNA (Zfp143 Mouse)
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR/hBIG1

    Plasmid

    #226251

    Purpose

    Entry plasmid of hBIG1 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Entry vector

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR/HA-hBIG2

    Plasmid

    #226252

    Purpose

    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Entry vector

    Tags

    HA

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti puro HA-VIM

    Plasmid

    #227585

    Purpose

    Lentiviral plasmid expressing human VIM protein

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    VIM (VIM Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

    Plasmid

    #225432

    Purpose

    Transcriptional unit of BeYDV Rep/RepA (C1:C2)

    Depositor

    Diego Orzaez

    Insert

    pNOS:Rep/RepA:tNOS
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEN_TT DNM1L-V5

    Plasmid

    #220359

    Purpose

    Gateway entry vector for an inducible V5-tagged DNM1L

    Depositor

    Kevin Janes

    Insert

    DNM1L (DNM1L Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    V5

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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