We narrowed to 5,521 results for: cycs

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pSAM2_mCherry_Mesp2

Plasmid

#72693

Purpose

Doxycyline regulated expression of Mesp2 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Mesp 2 (Mesp2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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integrin beta6 777T pcDNA1 neo

Plasmid

#13582

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Expression

Mammalian

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

Plasmid

#157692

Purpose

mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

Plasmid

#179428

Purpose

Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-(nospacer)-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDN-G1TCbt

Plasmid

#44515

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Plasmid

#165492

Purpose

Neuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

Use

AAV

Tags

darkVenus

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-LITAF

Plasmid

#178444

Purpose

doxycycline-inducible expression of Human LITAF in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

LITAF (LITAF Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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plko-tet-on-puromycin-shTRX1-259

Plasmid

#110920

Purpose

To knockdown Thioredoxin-1 following doxycycline addition

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Lentiviral

Promoter

unknown

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/SH/GW-Mst2

Plasmid

#84300

Purpose

Expresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst2. Used in conjunction with Flp-In cells to generate single copy transgene.

Depositor

Jonathan Chernoff

Article

Rawat et al J Biol Chem. 2013 Mar 22;288(12):8762-71

Insert

Mst2 (STK3 Human)

Tags

HA, SBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-bio-myc-NES-EGFP

Plasmid

#112712

Purpose

For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

EGFP

Tags

HIV Rev nuclear localization signal (NES), biotin…

Expression

Mammalian

Mutation

Nucleotide sequence optimized for synthetic gene …

Promoter

CMV with Tet repressor binding sites

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00273

Plasmid

#34401

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11987 (tgo Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUC19-F4GDP9

Plasmid

#103077

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

F4GDP9(Cas9 coding gene from Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 W999A pcDNA1 neo

Plasmid

#13589

Depositor

Dean Sheppard

Article

Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

Insert

integrin alpha 9 W999A (ITGA9 Human)

Expression

Mammalian

Mutation

W999A

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239604

Purpose

Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-Dox-AIO-TetOn_ETV2_Down-Tandem

Plasmid

#241393

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ETV2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-ETV2; rtTA-T2A-mTagBFP2 (ETV2 Human)

Tags

NLS

Expression

Mammalian

Promoter

TRE; CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pINDUCER20-ABL2(K281M)

Plasmid

#215470

Purpose

Dox-inducible lentiviral transfer vector with Arg kinase-dead (K281M)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ABL2 (ABL2 Human)

Use

Lentiviral

Mutation

K281M (kinase-inactivating)

Promoter

mCMV, Doxycycline-inducible

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-Fully-spliced CRNDE

Plasmid

#242178

Purpose

Express doxycycline inducible Fully-spliced CRNDE in mammalian cells

Depositor

Joshua Mendell

Article

Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

Insert

Fully-spliced CRNDE (CRNDE Human)

Expression

Mammalian

Mutation

PAM mutation (CCT to GTT)

Promoter

TRE3GS

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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