We narrowed to 7,046 results for: 11

1. pSPL3-hRPH3A_c.1853Gmut

   Plasmid

   #190479

   Purpose

   minigene assay

   Depositor

   Alfredo Brusco

   Article

   Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

   Insert

   RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.1853A>G

   Promoter

   SV40

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #128587

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

   Plasmid

   #114081

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

   Plasmid

   #159630

   Purpose

   AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

   Depositor

   Andrew Allen, Ross Bathgate

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   AAV

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Mutation

   Promoter

   DCA (same as CAG promoter)

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. anti-Jagged_D5-pBIOCAM5

   Plasmid

   #39345

   Depositor

   John McCafferty

   Article

   Schofield et al Genome Biol. 2007;8(11):R254.

   Insert

   scFv specific for Jagged (clone D5) (JAG1 Human)
   

   Use

   Tags

   3xFLAG and 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

   Plasmid

   #114075

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   E174R, S542R, K548V and N552R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAHISPApqsE

   Plasmid

   #97019

   Purpose

   Expressing Pseudomonas aeruginosa pqsE protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Quinolone signal response protein
   

   Use

   Tags

   N-ter TEV protease cleavable 6HIs

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEAHISMRSA2676

   Plasmid

   #96970

   Purpose

   expression of methicillin-resistant Staphylococcus aureus orf 2676

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Sar2676
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEAHISMRSAMVAK

   Plasmid

   #97000

   Purpose

   expression of methicillin-resistant Staphylococcus aureus MVAK protein

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Mevalonate kinase
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEAHISPAFabH

    Plasmid

    #97018

    Purpose

    Expressing Pseudomonas aeruginosa FabH protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    3-Oxoacyl-[acyl-carrier-protein] synthase III
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCRISPR v2-sgPlscr3-2#

    Plasmid

    #234772

    Purpose

    knockout Plscr3

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Plscr3 (Plscr3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-sgPlscr3-1#

    Plasmid

    #234767

    Purpose

    knockout Plscr3

    Depositor

    Judy Lieberman

    Article

    Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

    Insert

    Plscr3 (Plscr3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF_his6_sumo_ZIKV_NS5_MTase

    Plasmid

    #213529

    Purpose

    Expresses the MTase domain of NS5 of ZIKV

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    ZIKA virus NS5 methyltransferase domain
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    MTase domain aa 1-265

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Loxp-CMV-Loxp-GFP-LA

    Plasmid

    #206199

    Purpose

    Expresses GFP-lamin-A only in non-myocyte when delivered into mice carrying a cardiomyocyte-specific Myh6-Cre transgene

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Lmna (LMNA Human)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSPL3-hRPH3A_c.444Tmut

    Plasmid

    #190477

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.444G>T

    Promoter

    SV40

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEAHISSIRV131

    Plasmid

    #97053

    Purpose

    Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    SIRV orf 131
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEAHISSSO2462

    Plasmid

    #97064

    Purpose

    Expressing Sulfolobus solfataricus orf SSO2462 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus orf SSO2462
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEAHISSSO2452

    Plasmid

    #97059

    Purpose

    Expressing Sulfolobus solfataricus orf SSO2452 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus orf SSO2452
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEAHISPSVORF126

    Plasmid

    #97021

    Purpose

    Expressing Pyrobaculum spherical virus orf126 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    PSV orf 126
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEAHISPA5006

    Plasmid

    #97016

    Purpose

    Expressing Pseudomonas aeruginosa orf PA5006 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    P aeruginosa orf PA5006
    

    Use

    Tags

    N-ter TEV protease cleavable 6HIs

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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