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GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.FLP-FRT.iGluSnFR3.v857.SGZ

Plasmid

#196224

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in SGZ backbone. iGluSnFR3.v857.SGZ

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV hSyn FLP-FRT iGluSnFR3 v857.SGZ

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and Stargazin and PDZ dom…

Expression

Mammalian

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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1479 pcDNA3 flag HA 14-3-3 gamma

Plasmid

#9000

Depositor

William Sellers

Insert

14-3-3 gamma (Ywhag Rat)

Tags

HA and flag

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP-N1-VAPA(1-242)

Plasmid

#18874

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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cacna2d1 pCAGGS

Plasmid

#206104

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit

Depositor

Annette Dolphin

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

Plasmid

#102935

Purpose

An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

Depositor

Brandon Harvey

Article

Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

Insert

NK1R (Tacr1 Rat)

Use

AAV

Expression

Mammalian

Promoter

CMV-IE

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria2-GFP KI

Plasmid

#131490

Purpose

Endogenous tagging of GluA2: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria2, Gria2 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pFsynW KDEL Hook

Plasmid

#197277

Purpose

Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Streptavidin

Use

Lentiviral

Tags

FLAG

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHes1-DN-GFP (CC#110)

Plasmid

#15134

Depositor

Connie Cepko

Article

Furukawa et al Neuron. 2000 May . 26(2):383-94.

Insert

Hes1 (Hes1 Rat)

Use

Retroviral

Expression

Mammalian

Mutation

E43A, K44A, R47A

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-synp-F-synaptophysin-GCaMP6f

Plasmid

#102996

Purpose

Expresses synaptophysin fused GCaMP6f under the synapsin promoter

Depositor

David Cox

Insert

Synaptophysin-GCaMP6f

Use

AAV

Expression

Mammalian

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jGCaMP8m

Plasmid

#172719

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Nlgn3 KI

Plasmid

#131501

Purpose

Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Nlgn3, Nlgn3 Mouse, Rat)

Use

CRISPR

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCzeta

Plasmid

#10799

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pORANGE GFP-Syt7 KI

Plasmid

#131488

Purpose

Endogenous tagging of Synaptotagmin-7: N-terminal (amino acid position: P5)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Syt7, Syt7 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP7c variant 1513-WPRE

Plasmid

#105321

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV1

Insert

jGCaMP7c variant 1513

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-citrine

Plasmid

#50971

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

Synaptophysin-miniSOG-citrine (Syp Rat)

Use

AAV

Tags

citrine and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV5 alpha 1A AR

Plasmid

#45760

Depositor

Robert Lefkowitz

Article

Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

Insert

alpha 1A AR (Adra1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
mTagBFP-Lysosomes-20

Plasmid

#55263

Purpose

Localization: Lysosome Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LAMP1

Tags

mTagBFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRB133

Plasmid

#180176

Purpose

GluN2A in pCDNA3.1

Depositor

Richard Bergeron

Article

Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

Insert

GluN2A (Grin2a Rat)

Expression

Mammalian

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-hPGK-eGFP-SynaptoZip

Plasmid

#122523

Purpose

Lentiviral expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by hPGK promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

Lentiviral

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hPGK

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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