2,293 results

1. AAV-SYP1-miniSOG-citrine

   Plasmid

   #50971

   Purpose

   AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   Synaptophysin-miniSOG-citrine (Syp Rat)
   

   Use

   AAV

   Tags

   citrine and miniSOG

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-hSyn-Axon-jGCaMP8m

   Plasmid

   #172719

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #50942

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Tobias Bonhoeffer, Tobias Rose, Mark Huebener

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-Actb KI #2

   Plasmid

   #139666

   Purpose

   Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-mEGFP-CaMKIIa (T286A)

   Plasmid

   #127390

   Purpose

   Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   pCAG

   Available Since

   Jan. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE unc13a GFP KI

   Plasmid

   #131498

   Purpose

   Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Unc13a Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FU- dio Teal-gephyrin - W

   Plasmid

   #73918

   Purpose

   To express the inhibitory scaffold gephyrin tagged to teal fluorescent protein. Expression is Cre dependant. Plasmid can also be used to make lentivirus.

   Depositor

   Elly Nedivi

   Article

   Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

   Insert

   Teal-gephyrin
   

   Use

   Lentiviral

   Tags

   Teal

   Expression

   Mammalian

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNAI-GAL4-CREB-S133A

   Plasmid

   #46770

   Purpose

   Expression of GAL4 fusion to CREB-S133A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S133A (Creb1, GAL4 Budding Yeast, Rat)
   

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 133 to Alanine

   Promoter

   CMV

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNAI-GAL4-CREB-S142A

   Plasmid

   #46771

   Purpose

   Expression of GAL4 fusion to CREB-S142A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S142A (Creb1, GAL4 Budding Yeast, Rat)
   

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 142 to Alanine

   Promoter

   CMV

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE Gria2-GFP KI

    Plasmid

    #131490

    Purpose

    Endogenous tagging of GluA2: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria2, Gria2 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD200-Cd4d3+4-COMP-blac-3xFLAG-6his

    Plasmid

    #71471

    Purpose

    A positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    CD200 (Cd200 Rat)
    

    Tags

    6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGADT7-αC

    Plasmid

    #197650

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-2 αC
    

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Ser677T substitution

    Promoter

    ADH1

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. G protein alpha-s-CFP

    Plasmid

    #55793

    Purpose

    G protein alpha-s internally tagged with ECFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-ECFP (Gnas Rat)
    

    Tags

    ECFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    His was substituted for Asn164 in ECFP (Clontech)…

    Promoter

    CMV

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCit-4aa-SNAP25B

    Plasmid

    #53235

    Purpose

    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    SNAP-25B (Snap25 Rat)
    

    Tags

    4aa-linker and mCitrine

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid

    #55797

    Purpose

    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

    Promoter

    CMV

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W

    Plasmid

    #32520

    Depositor

    Mustafa Sahin

    Article

    Nie et al Nat Neurosci. 2010 Feb;13(2):163-72. Epu

    Insert

    Constitutively active Rheb (S16H) (Rheb Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S16H to make constitutively active

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAX DAB1

    Plasmid

    #30139

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Disabled-1 (Dab1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. mEmerald-Fidgetin-C-18

    Plasmid

    #62756

    Purpose

    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Fidgetin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGP-CMV-NEW-fRGECO2

    Plasmid

    #125248

    Purpose

    Expresses red fluorescent calcium sensor in mammalian cells

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRGECO2
    

    Tags

    NES signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-mDlx-jGCaMP8m-WPRE

    Plasmid

    #176754

    Purpose

    jGCaMP8m expression in GABA-ergic interneurons under the control of mDlx enhancer

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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