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Plasmid#196933PurposeSunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.DepositorInsertRenilla luciferase
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRNA1-OE-METTL16-sgMETTL16-MUT2
Plasmid#196202Purposeoverexpress METTL16DepositorInsertSynonymous mutation of METTL16 ORF (METTL16 Human)
UseLentiviralTagsExpressionMutationSynonymous mutationPromoterAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-DVL1-MUT
Plasmid#194587PurposeMammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion ProteinDepositorInsertDVL1 (DVL1 Human)
UseTagsmEGFPExpressionMammalianMutationH502Pfs*143PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-SOX2-MUT
Plasmid#194581PurposeMammalian Expression of mEGFP-SOX2 Mutant (M276Ifs*95) Fusion ProteinDepositorInsertSOX2 (SOX2 Human)
UseTagsmEGFPExpressionMammalianMutationM276Ifs*95PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-CALR-MUT
Plasmid#194579PurposeMammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion ProteinDepositorInsertCALR (CALR Human)
UseTagsmEGFPExpressionMammalianMutationD387Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_1 KLF2mut 449,899
Plasmid#194198PurposeIncludes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,899)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation2 mutated KLF binding sites (position 449,899)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_1 KLF2mut 449,548
Plasmid#194199PurposeIncludes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,548)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation2 mutated KLF binding sites (position 449,548)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_1 KLF2mut 449,732
Plasmid#194200PurposeIncludes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,732)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation2 mutated KLF binding sites (position 449,732)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_1 KLF2mut 449,884
Plasmid#194201PurposeIncludes the promoter (1kb) of SMTS with 2 mutated KLF binding sites (position 449,884)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation2 mutated KLF binding sites (position 449,884)PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-ZNF354C_mutant_M41K-L42K-E43K
Plasmid#194185PurposeExpresses ZNF354C with mutated KRAB domain (M41K, L42K, E43K mutation)DepositorInsertZNF354C (ZNF354C Human)
UseTagsMyc-DDKExpressionMammalianMutationKRAB domain mutant M41K/L42K/E43KPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)
Plasmid#194191PurposeIncludes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation4 mutated KLF binding sites (positions 449,548,73…PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732 (Tri)
Plasmid#194192PurposeIncludes the promoter (1kb) of SMTS with 3 mutated KLF binding sites (positions 449,548,732)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseTagsExpressionMutation3 mutated KLF binding sites (positions 449,548,73…PromoterSMANTIS promoter (1kb), mutatedAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsRCK-mut1_O
Plasmid#147077PurposeMammalian Expression of HsRck-mut1DepositorInsertHsRck-mut1 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-mut1_N
Plasmid#147023PurposeMammalian Expression of HsRck-mut1DepositorInsertHsRck-mut1 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-Nterm6Wmut_O
Plasmid#147068PurposeInsect Expression of DmGW182-Nterm6WmutDepositorInsertDmGW182-Nterm6Wmut (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TAPtagN-HsRCK-mut1_K
Plasmid#146727PurposeMammalian Expression of HsRck-mut1DepositorInsertHsRck-mut1 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-NTAP-DmMe31B-mut1_L
Plasmid#146818PurposeInsect Expression of DmMe31B-mut1DepositorInsertDmMe31B-mut1 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-P-HsRCK_296-472-mut_E
Plasmid#146172PurposeBacterial Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)-MutTER
Plasmid#178035PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deleted and Changed T(8…PromoterT7LacIAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16 E7 Mutant 2
Plasmid#191546PurposeProtein expression of HPV16 E7 Mutant 2DepositorInsertHPV16
UseRetroviralTagsFlagHAExpressionMutationI93A, K97A, P98APromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-P C-FlagHA HPV16 E7 Mutant 1
Plasmid#191545PurposeProtein expression of HPV16 E7 Mutant 1DepositorInsertHPV16
UseRetroviralTagsFlagHAExpressionMutationR49A, H51A, H73A, E80A, D81APromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2-5xmut_V
Plasmid#147806PurposeMammalian Expression of HsDCP2-5xmutDepositorInsertHsDCP2-5xmut (DCP2 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence gi…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-HUWE1_UBA/UIM-mut
Plasmid#187151PurposeEntry cloneDepositorInsertHUWE1 (HUWE1 Human)
UseEntry cloneTagsExpressionMutationM1328A/F1330A/A1376G/S1382APromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q
Plasmid#147338PurposeInsect Expression of DmDCP1-GSSGmut-dsRNAresDepositorInsertDmDCP1-GSSGmut-dsRNAres (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Mcm2-2A mutation sgRNA
Plasmid#186936PurposesgRNA used for genetic mutation at Y81 and Y90 of Mcm2DepositorInsertMcm2-2A mutation sgRNA (Mcm2 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Plekhh1 5 silent mutations
Plasmid#187373PurposeExpresses human Plekhh1 with 5 silent mutations labelled with CherryDepositorInsertPlekhh1 with 5 silent mutations
UseTagsmCherryExpressionMammalianMutationFive silent mutations at the Plekhh1 siRNA site (…PromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-PPK(mutant)
Plasmid#183942PurposeExpresses catalytic mutant of E. coli PPK from yeast GPD promoterDepositorInsertPPK(mutant) (ppk E. coli )
UseTagsExpressionYeastMutationmutations in His435, His454, and His592 to AlaninePromoterGPDAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-HA-PPK(mutant)
Plasmid#183944PurposeExpresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoterDepositorInsertPPK(mutant) (ppk E. coli)
UseTagsHAExpressionYeastMutationmutations in His435, His454, and His592 to AlaninePromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA T7-Gnat1 Mut-3UTR
Plasmid#184027PurposeExpresses mouse Gnat1 protein with N-terminal T7 tag from cDNA that contains the mutant 3'-UTR where the TAG binding sites for MSI1 are mutated to TGADepositorInsertGnat1 (Gnat1 Mouse)
UseTagsT7ExpressionMammalianMutationTAG sites in the 3'-UTR are mutated to TGAPromoterCMVAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-6xmut_U
Plasmid#147732PurposeMammalian Expression of HsNot1iso2-6xmutDepositorInsertHsNot1iso2-6xmut (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only