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pAT15516_BEAR-GFP-2in1
Plasmid#174082PurposeBEAR-GFP plasmid with a gRNA that targets its own plasmidDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCB578
Plasmid#141095PurposeE. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat arrayDepositorInsertsCas9 and tracrRNA
repeat-spacer-repeat array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cre)
Plasmid#174862PurposeExpresses mouse Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by MmPeg10 UTRs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Hs.cargo(Cre)
Plasmid#174863PurposeExpresses human Peg10 cargoRNA encoding Cre recombinaseDepositorInsertCre flanked by HsPeg10 UTRs
UseTagsSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Blast_DEST mouse_p65promoter_mouse_p65-mRuby2
Plasmid#124903PurposeLentiviral vector to express p65-mRuby2 under p65 promoter (With blasticidin resistance)DepositorInsertp65 promoter- p65 CDS-mRuby2
UseLentiviralTagsmRubyExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-FP4-mito
Plasmid#229692PurposeTransient expression of EGFP FP4-mito in mammalian cellsDepositorInsertEGFP; Mitochondira FP4 motifs from Listeria ActA
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-mito
Plasmid#229693PurposeTransient expression of EGFP AP4-mito in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (mitochondria)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAT9651-BEAR-GFP
Plasmid#162989PurposeBEAR target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9752-BEAR-mScarlet
Plasmid#162991PurposeBEAR target plasmid with split mScarlet and disrupted 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-MCS-NLS-3XFLAG-V5
Plasmid#196383PurposeEmpty backbone vector to insert cDNA for strong expression in mammalian cellsDepositorTypeEmpty backboneUseTags3xFLAG and V5ExpressionBacterial and MammalianMutationPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_rpsM_mCerulean3
Plasmid#124904PurposerpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)DepositorInsertrpsM promoter and mCerulean3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc CDC4 R465C
Plasmid#16653DepositorInsertCDC4 R465C (FBXW7 Human)
UseTagsmycExpressionMammalianMutationR465CPromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
RnBarr2-mCherry
Plasmid#137794PurposeVisualization of beta-arrestin2DepositorInsertRat beta-arrestin2 (Arrb2 Rat)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsGRK6-mVenus
Plasmid#137780PurposeVisualization of GRK6DepositorInsertHuman GRK6 (GRK6 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT9650-BEAR-GFP-preedited
Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp9 C287A
Plasmid#11819DepositorInsertCaspase 9 catalytically incompetent mutant (CASP9 Human)
UseTagsExpressionMammalianMutationC287A, catalytically incompetent mutant.PromoterAvailable sinceMay 12, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHJ4: LexA-VHH-Caffeine
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
