We narrowed to 31,398 results for: Eng

1. pET30b_T7Sav

   Plasmid

   #138588

   Purpose

   T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   T7-tagged streptavidin variant with native C-terminus
   

   Use

   Synthetic Biology

   Tags

   T7-tag

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-T7Max-UTR1-deGFP-8xHis-T500

   Plasmid

   #178422

   Purpose

   Expression of deGFP via a T7 Promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Insert

   deGFP
   

   Use

   Synthetic Biology

   Tags

   8xHis Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7Max

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCORE-Kp53

   Plasmid

   #72241

   Purpose

   Contains the CORE cassette with KanMX4 and human p53 gene markers

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   kanMX4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V122A mutation

   Promoter

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBTRCk-pVhb-maACR

   Plasmid

   #215651

   Purpose

   pBBR1 Origin, KanR, with maACR gene under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   acyl-CoA reductase
   

   Use

   Tags

   Maltose binding protein and His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Vhb

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-pVhb-vfFadBA

   Plasmid

   #215650

   Purpose

   pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Vhb

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY2

   Plasmid

   #218115

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY3

   Plasmid

   #218116

   Purpose

   Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY3-LYS-IV

   Plasmid

   #218119

   Purpose

   LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

   Depositor

   Carl Denard

   Article

   Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNVLTv2-pvdIJD-Ptrc-base

   Plasmid

   #169243

   Purpose

   Suicide vector for integrating expression cassette in P. putida (IPTG induction)

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Insert

   lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-Casp2

    Plasmid

    #50023

    Purpose

    pENTR vector for mouse Caspase-2

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    Casp2 (Casp2 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PJ23119

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH613

    Plasmid

    #169602

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4 / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH232-Tier1-PmPGK1-mRuby2

    Plasmid

    #169523

    Purpose

    Tier-1 vector encoding PmPGK1-driven mRuby2 expression (PmPGK1-mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas2F

    Plasmid

    #82377

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMM545-Tier1-PhCMV-Citrine

    Plasmid

    #169505

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein Citrine (PhCMV-Citrine-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven yellow fluorescent protein citrine
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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