We narrowed to 31,835 results for: Eng;

1. pRC3EGFP

   Plasmid

   #193790

   Purpose

   Expresses EGFP under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   EGFP
   

   Expression

   Bacterial

   Promoter

   PR promoter

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVH607-Tier1-PCREm4-Nluc

   Plasmid

   #169594

   Purpose

   Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH231-Tier1-PmPGK1-mTagBFP2

   Plasmid

   #169520

   Purpose

   Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven monomeric Tag blue fluorescent protein 2
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTCW012

   Plasmid

   #83527

   Purpose

   Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   pFUS1J2

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH233-Tier1-PmPGK1-eYFP

   Plasmid

   #169522

   Purpose

   Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven enhanced yellow fluorescent protein
   

   Expression

   Mammalian

   Promoter

   PPGK

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30b_T7Sav

   Plasmid

   #138588

   Purpose

   T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   T7-tagged streptavidin variant with native C-terminus
   

   Use

   Synthetic Biology

   Tags

   T7-tag

   Expression

   Bacterial

   Promoter

   PT7

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCORE-Kp53

   Plasmid

   #72241

   Purpose

   Contains the CORE cassette with KanMX4 and human p53 gene markers

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Genet Eng (N Y). 2003;25:189-207.

   Inserts

   p53 (TP53 Human)
   kanMX4
   

   Expression

   Bacterial

   Mutation

   V122A mutation

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTRCk-pVhb-maACR

   Plasmid

   #215651

   Purpose

   pBBR1 Origin, KanR, with maACR gene under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   acyl-CoA reductase
   

   Tags

   Maltose binding protein and His tag

   Expression

   Bacterial

   Promoter

   Vhb

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACYC-pVhb-vfFadBA

   Plasmid

   #215650

   Purpose

   pACYC Origin, CmR, with vfFadBA gene under Vhb promoter

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Insert

   thiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
   

   Expression

   Bacterial

   Promoter

   Vhb

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY2

    Plasmid

    #218115

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY3

    Plasmid

    #218116

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY3-LYS-IV

    Plasmid

    #218119

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Expression

    Bacterial

    Promoter

    lacIq

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-Casp2

    Plasmid

    #50023

    Purpose

    pENTR vector for mouse Caspase-2

    Depositor

    Jamie Davies

    Article

    Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

    Insert

    Casp2 (Casp2 Mouse)
    

    Use

    Entry vector

    Available Since

    Dec. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Expression

    Bacterial

    Mutation

    V122A mutation

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTCON2-GFP-TAG

    Plasmid

    #158131

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Expression

    Yeast

    Mutation

    Introduced a stop codon in place of the tyrosine …

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas5

    Plasmid

    #82378

    Purpose

    sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PJ23119

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFOX6-Tier1-PhCMV-TdTomato

    Plasmid

    #169515

    Purpose

    Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven dimeric red fluorescent protein TdTomato
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVH613

    Plasmid

    #169602

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Expression

    Mammalian

    Promoter

    PCREm4 / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS416-Aga1p-FAPB2.3.6

    Plasmid

    #158147

    Purpose

    Yeast display reporter for non-display yeast strains (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    Aga1p-FAPB2.3.6
    

    Expression

    Yeast

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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