We narrowed to 1,606 results for: Sp1

1. pCG_batCoV RaTG13 NSP16 C-V5-tag

   Plasmid

   #179985

   Purpose

   Mammalian expression vector for RaTG13 Nsp16, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   RaTG13 Nsp16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCG_SARS-CoV1 NSP16 C-V5-tag

   Plasmid

   #179965

   Purpose

   Mammalian expression vector for SARS-CoV-1 Nsp16, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-1 Nsp16 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQ

   Plasmid

   #168152

   Purpose

   Inducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

   Plasmid

   #168134

   Purpose

   Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p28BIOHTEV-LIC: nsp10_SARS-CoV-2

   Plasmid

   #167847

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp10

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp10
   

   Use

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 1, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA-HisFlag-rUSP19ER-CAmut

   Plasmid

   #155237

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Article

   Lu et al Mol Cell Biol. 2009 Jan;29(2):547-58. do

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Ala

   Promoter

   CMV

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-HisFlag-rUSP19ER-CRmut

   Plasmid

   #155253

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) CR mutant (Usp19 Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Cys(545) to Arg

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-HisFlag-rUSP19ER deltaN3

   Plasmid

   #155243

   Purpose

   for mammalian overexpression of truncated (delta1-1631) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-1631) (ER isoform) (Usp19 Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-HisFlag-rUSP19ER deltaN2

   Plasmid

   #155242

   Purpose

   for mammalian overexpression of truncated (delta1-1088) ER isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (delta1-1088) (ER isoform) (Usp19 Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-HisFlag-rUSP19ER deltaN1

    Plasmid

    #155241

    Purpose

    for mammalian overexpression of truncated (delta1-536) ER isoform of rat USP19

    Depositor

    Simon Wing

    Insert

    USP19 (delta1-536) (ER isoform) (Usp19 Rat)
    

    Use

    Tags

    6XHis-3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. HSP104-b/Leu(G217S/T499I)

    Plasmid

    #1158

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    HSP104 (HSP104 Budding Yeast)
    

    Use

    Tags

    GAL1:10 promoter

    Expression

    Yeast

    Mutation

    G217S/T499I

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

12. NaVSp1 Bh1 CTD pIRES

    Plasmid

    #128080

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-220 NaVBh1 236-274) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NavSp1_Bh1CTD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. NaVSp1 Ab1 CTD pIRES

    Plasmid

    #128082

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-220 NaVAb1 234-283) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NavSp1_Ab1CTD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. NaVAe1 Sp1 CTD pIRES

    Plasmid

    #128085

    Purpose

    Mammalian cell expression vector for (NaVAe1 1-243 NaVSp1 220-255) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVAe1 and Sp1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-sumo-NSP12 (SARS-CoV-2)

    Plasmid

    #159107

    Purpose

    The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

    Depositor

    Thomas Tuschl

    Article

    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

    Insert

    NSP12
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR207 SARS-CoV-2 NSP1 Delta RC

    Plasmid

    #164522

    Purpose

    Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Insert

    NSP1 Delta RC (ORF1ab )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Insert of NSP1 gene's CDS from SARS-CoV-2 is…

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)

    Plasmid

    #169188

    Purpose

    To express His-SUMO-nsp12 (SARS-CoV-2) in E. coli

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    14His-SUMO-nsp12 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO (Ulp1-cleavable)

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5953 pcDNA-Xpress-His-USP15

    Plasmid

    #23216

    Depositor

    Peter Howley

    Article

    Vos et al J Virol. 2009 Sep . 83(17):8885-92.

    Insert

    USP15 (USP15 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJL44 - Phsp16.48::MosTase::glh-2utr

    Plasmid

    #19333

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Phsp-16::Mos1 transposase
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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