We narrowed to 1,270 results for: grna cloning vector

1. pMOD_B-Pho2-1/2-2-Csy4

   Plasmid

   #161760

   Purpose

   MODULE B Csy4 vector with 6x gRNAs targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pMOD_B2103 - #91061)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   gRNAs
   

   Expression

   Bacterial

   Promoter

   CmYLCV Promoter

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. JD633

   Plasmid

   #160393

   Purpose

   CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots CRISPR experiments. Includes two AarI sites for gRNA cloning

   Depositor

   Jorge Dubcovsky

   Article

   Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

   Inserts

   ZmUbi::wheat GRF4-GIF1 chimera
   ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   Binary vector

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMZ379

   Plasmid

   #74215

   Purpose

   Combined sgRNA/Cas9 vector with NatMX marker

   Depositor

   Mikel Zaratiegui

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ377

   Plasmid

   #74214

   Purpose

   Combined sgRNA/Cas9 vector with LEU2 marker

   Depositor

   Mikel Zaratiegui

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiCRISPRv2-mCherry

   Plasmid

   #99154

   Purpose

   Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

   Depositor

   Agata Smogorzewska

   Inserts

   Cas9
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Aug. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMEL13

   Plasmid

   #107919

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMEL12

   Plasmid

   #107918

   Purpose

   hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMEL16

   Plasmid

   #107922

   Purpose

   HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMEL10

   Plasmid

   #107916

   Purpose

   URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMEL17

    Plasmid

    #107923

    Purpose

    TRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMEL15

    Plasmid

    #107921

    Purpose

    nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMEL14

    Plasmid

    #107920

    Purpose

    LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEL11

    Plasmid

    #107917

    Purpose

    amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ376

    Plasmid

    #74213

    Purpose

    Combined sgRNA/Cas9 vector with KanMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEn-Chimera

    Plasmid

    #61432

    Purpose

    Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP003

    Plasmid

    #101164

    Purpose

    E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRB1017

    Plasmid

    #59936

    Purpose

    empty vector for gRNA cloning

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJEC625

    Plasmid

    #174368

    Purpose

    Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJF131B

    Plasmid

    #113326

    Purpose

    pTet-W108 scRNA.b2, pTet-RR2-sgRNA

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    W108 scRNA_MS2 and sgRNA_RR2
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB_p300_core-dCas9-EGFP_hygro

    Plasmid

    #226431

    Purpose

    PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker

    Depositor

    Alon Goren

    Inserts

    p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    HA tag, P2A-EGFP, and p300 core

    Expression

    Mammalian

    Promoter

    Dox inducible minimal CMV and UbC Promoter

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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