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pcDNA5-F-AF9

Plasmid

#49428

Purpose

Mammalian expression of Flag-tagged human AF9

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

AF-9 (MLLT3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Narg2

Plasmid

#49247

Purpose

mammalian expression of flag-tagged human Narg2

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Narg2 (NARG2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KA

Plasmid

#133582

Purpose

Expresses IP3KA GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only
pFUPI hT5-S322-Cyp

Plasmid

#80282

Purpose

Expresses human TRIM5 CypA fusion

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion (TRIM5, PPIA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Blunt-U6-HES7-STOP-gRNA

Plasmid

#130933

Purpose

gRNA for tag human HES7

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7-STOP-gRNA (HES7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFUPI hT5Cyp-H126Q

Plasmid

#80281

Purpose

Expresses human TRIM5 CypA fusion with H126Q mutation in CypA

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion (TRIM5, PPIA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

H126Q mutation in CypA

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET42b-A3A-PBE-ΔUGI

Plasmid

#119771

Purpose

To express the recombinant APOBEC3A in vitro

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS

Use

Tags

6*HIS and NLS

Expression

Bacterial

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEN017-SpCas9-hiNLS-t-M4

Plasmid

#234455

Purpose

Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS5A

Plasmid

#17646

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS5A (POLY Hepatitis C virus)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag1-NS3

Plasmid

#17645

Depositor

Xin Wang

Article

Budhu et al Carcinogenesis. 2007 Jul . 28(7):1552-60

Insert

HCV NS3 (POLY Hepatitis C virus)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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GID4-Nluc

Plasmid

#200879

Purpose

Mammalian expression of human GID4 with C-terminal NanoLuciferase fusion

Depositor

Stefan Knapp

Article

Owens et al bioRxiv 2023.01.17.524225

Insert

GID4-NanoLuciferase (C17orf39 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA-hTtAgo

Plasmid

#104383

Purpose

human codon optimized TtAgo; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al PLoS One. 2018 Mar 27;13(3):e0193818. do

Insert

hTtAgo

Use

Tags

3XFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTHRB-donor

Plasmid

#112333

Purpose

CRISPR donor plasmid to tag human transcription factor THRB with GFP

Depositor

Kevin White

Insert

THRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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OTUB1/pRetroX-IRES-DsRed#10

Plasmid

#180279

Purpose

Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

human Otubain-1 (OTUB1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLI_TNF

Plasmid

#171179

Purpose

inducible expression of human TNF

Depositor

Veit Hornung

Article

Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

Insert

human TNF

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEF2-FL-hMer_gR1

Plasmid

#225993

Purpose

Expresses a chimeric contruct having N-terminal human MerTK and C-terminal human Interferon Gamma Receptor

Depositor

Sergei Kotenko, Raymond Birge

Article

Tsou et al J Biol Chem. 2014 Sep 12;289(37):25750-6

Insert

human MerTK N-Terminal human Interferon Gamma R1 Receptor C-Terminal fusion (MERTK, IFNGR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEF2-EFGR-hAxl

Plasmid

#225997

Purpose

Expresses a chimeric contruct having N-terminal human EGFR and C-terminal human Axl

Depositor

Sergei Kotenko, Raymond Birge

Article

Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

Insert

human EGFR N-Terminal human Axl C-Terminal Fusion (EGFR, AXL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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