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TDP43-NTD

Plasmid

#241024

Purpose

Bacterial expression of TDP-43 NTD

Depositor

Nicolas Fawzi

Publication

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP43-NTD

Tags

6x His

Expression

Bacterial

Mutation

changed N81 to stop codon

Available since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV

Plasmid

#235547

Purpose

To express GFP

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Type

Empty backbone

Use

Lentiviral

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Publication

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEPCTαKN0005

Plasmid

#187569

Purpose

Transcriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoter

Depositor

Nicola Patron

Publication

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Luciferase

Expression

Plant

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0750

Plasmid

#185627

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

Depositor

Nicola Patron

Publication

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Publication

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ Lifeact-mCherry

Plasmid

#185408

Purpose

Red fluorescent reporter for polymerized actin. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Publication

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

lifeact

Use

In vitro synthesis of mrna

Tags

mCherry

Expression

Mammalian

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTHMT_hnRNPF_FL

Plasmid

#139114

Purpose

express FL hnRNPF with N-terminal maltose binding protein tag

Depositor

Nicolas Fawzi

Publication

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPF (HNRNPF Human)

Expression

Bacterial

Mutation

None

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0416

Plasmid

#154573

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Publication

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_110

Use

Synthetic Biology

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPKK0CM0068

Plasmid

#154596

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Publication

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

TALE

Use

Synthetic Biology

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0410

Plasmid

#154567

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Publication

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_104

Use

Synthetic Biology

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0411

Plasmid

#154568

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Publication

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_105

Use

Synthetic Biology

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC0CM0414

Plasmid

#154571

Purpose

Phytobricks Level 0 part

Depositor

Nicola Patron

Publication

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_108

Use

Synthetic Biology

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGI3EM09

Plasmid

#135482

Purpose

Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

Depositor

Nicolas Buchler

Publication

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunHsp70pr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCk2

Plasmid

#136696

Purpose

Acceptor plasmid for odd level position 2, lacZ blue-white screening.

Depositor

Nicola Patron

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCk1

Plasmid

#136695

Purpose

Acceptor plasmid for odd level position 1, lacZ blue-white screening.

Depositor

Nicola Patron

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Fyn-SH3_5WT_S25C

Plasmid

#118824

Purpose

Bacterial expression of the SH3 domain of human Fyn

Depositor

Nicolas Fawzi

Publication

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

FYN (FYN Human)

Tags

6-His-TEV tag

Expression

Bacterial

Mutation

S25C; See depositor comments below.

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_266-341

Plasmid

#118821

Purpose

Bacterial expression of hnRNPA2 truncation

Depositor

Nicolas Fawzi

Publication

Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

His and TEV

Expression

Bacterial

Mutation

contains amino acids 266-341

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0013

Plasmid

#117549

Purpose

Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Publication

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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