We narrowed to 639 results for: pBR322

1. pCARGO-RMCE

   Plasmid

   #233264

   Purpose

   Backbone plasmid used to clone lncRNA inserts in preparation for RMCE

   Depositor

   Mauro Calabrese

   Article

   Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GT_CAG-ITPKA-mScarlet-I

   Plasmid

   #229778

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

   Plasmid

   #229793

   Purpose

   Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSD068F

   Plasmid

   #218137

   Purpose

   Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD36-eGFP-IRES2-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SUMO-Twinkle 43-684

   Plasmid

   #205056

   Purpose

   TWNK protein expression

   Depositor

   Smita Patel

   Article

   Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

   Insert

   TWNK (TWNK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks amino acid from 1-42

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SUMO-Twinkle 360-684

   Plasmid

   #205055

   Purpose

   TWNK protein expression

   Depositor

   Smita Patel

   Article

   Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

   Insert

   TWNK (TWNK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks N terminal amino acid from 1-359

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTF1 deltaC12

   Plasmid

   #205068

   Purpose

   MTF1 protein expression

   Depositor

   Smita Patel

   Article

   Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

   Insert

   MTF1 (MTF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks C-terminal 12 amino acids

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTF1 deltaC16

   Plasmid

   #205069

   Purpose

   MTF1 protein expression

   Depositor

   Smita Patel

   Article

   Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

   Insert

   MTF1 (MTF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks C-terminal 16 amino acids

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTF1 deltaC18

   Plasmid

   #205070

   Purpose

   MTF1 protein expression

   Depositor

   Smita Patel

   Article

   Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

   Insert

   MTF1 (MTF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks C-terminal 18 amino acids

   Promoter

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. MTF1 deltaC20

    Plasmid

    #205071

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 20 amino acids

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MTF1 deltaC9

    Plasmid

    #205067

    Purpose

    MTF1 protein expression

    Depositor

    Smita Patel

    Article

    Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

    Insert

    MTF1 (MTF1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks C-terminal 9 amino acids

    Promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AV13_pCAG.Cas9.gRNA.NT

    Plasmid

    #199260

    Purpose

    Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9 nuclease
    Non-targeting (NT) control gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. piggybac-EF1-halo-Oct4

    Plasmid

    #197909

    Purpose

    single-particle tracking for OCT4

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    pou5f1 (Pou5f1 Mouse)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-CVm-Sec22b

    Plasmid

    #168029

    Purpose

    Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-Sec22b(A131-H190)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-CVm-VAMP8

    Plasmid

    #168028

    Purpose

    Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-VAMP8(G7-R67)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDUAL_2_lacZ

    Plasmid

    #155173

    Purpose

    see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDUAL_2_tacI

    Plasmid

    #155174

    Purpose

    see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBICI_2_medium

    Plasmid

    #155167

    Purpose

    see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAL928

    Plasmid

    #119853

    Purpose

    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysteine 169 to Serine, Cysteine 179 to Serine, C…

    Promoter

    T7

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. YE1-BE4max-NG

    Plasmid

    #138159

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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