We narrowed to 1,648 results for: sfGFP

1. pLenti sfGFP-LAMP1-mCherry (pHLARE)

   Plasmid

   #164478

   Purpose

   Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

   Depositor

   Diane Barber

   Article

   Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   mCherry and superfolder GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

   Plasmid

   #141184

   Purpose

   Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Has Service

   Cloning Grade DNA

   Insert

   S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
   

   Use

   Tags

   Signal peptide from influenza HA and Superfolder …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TfR-sfGFP-myc tag-SpyCatcher003

   Plasmid

   #133451

   Purpose

   Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   TfR-sfGFP-myc tag-SpyCatcher003
   

   Use

   Tags

   GSSGS and myc tag

   Expression

   Mammalian

   Mutation

   Contains C20 and A23 mutations that improve plasm…

   Promoter

   CMV

   Available since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-PelB-6H-Ag43-160N-sfGFP

   Plasmid

   #172886

   Purpose

   Display sfGFP protein on E. coli cell surface

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   SfGFP
   Ag43, partial (flu )
   

   Use

   Tags

   HHHHHH and TEV recognition site (ENLYFQG)

   Expression

   Bacterial

   Mutation

   Deleted 1-160 (N terminal)

   Promoter

   T7 Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEVI(408)-ColE-iLight-msfGFP

   Plasmid

   #170268

   Purpose

   Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   iLight
   

   Use

   Tags

   msfGFP

   Expression

   Bacterial

   Mutation

   To design iLight for expression in bacteria, a fu…

   Promoter

   pJ23116

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

   Plasmid

   #158741

   Purpose

   Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Hemagglutinin A/MI/45/15 H1N1
   

   Use

   Tags

   GCN4-TEV-sfGFP-TwinStrep

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-PGK-PYL1-sfGFP-Coilin

   Plasmid

   #122177

   Purpose

   expression of PYL1-SfGFP-Coilin, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   Coilin (COIL Human)
   

   Use

   Lentiviral

   Tags

   PYL1-SFGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-TRE3G-PYL1-sfGFP-PML

   Plasmid

   #167011

   Purpose

   expression of PYL1-SfGFP-PML, lentivirus vector

   Depositor

   Stanley Qi

   Article

   Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

   Insert

   PML (PML Human)
   

   Use

   Lentiviral

   Tags

   GFP-PML

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b Ag43 160N 6H sfGFP-R5 AmpR

   Plasmid

   #225139

   Purpose

   Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

   Depositor

   Urartu Seker

   Article

   Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

   Insert

   Ag43 protein fused to silaffin-R5 and sfGFP
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-SFFV-PYL1-sfGFP-Emerin

    Plasmid

    #121532

    Purpose

    expression of PYL-SfGFP-Emerin, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    Emerin (EMD Human)
    

    Use

    Lentiviral

    Tags

    PYL1-SFGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKIBra-P2A-mCherry-Puro-sfGFP

    Plasmid

    #115686

    Purpose

    Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

    Depositor

    David Suter

    Article

    Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

    Insert

    P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCD5-H3TX12-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158739

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H3/TX/12 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/TX/50/12 H3
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    G186V S219F

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

    Plasmid

    #173452

    Purpose

    Contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

    Insert

    mCherry-sfGFP-ADH1ter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid

    #231549

    Purpose

    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    msfGFP (intramolecular)

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-tdMCP-msfGFP(V206K)-GBI

    Plasmid

    #205163

    Purpose

    express tdMCP-msfGFP(V206K)-GBI

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    tdMCP-msfGFP(V206K)-GBI
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBMN-FKBP DD(L106P)-UbK0G75S-sfGFP

    Plasmid

    #170157

    Purpose

    Expression for LIBRON constructs

    Depositor

    Thomas Wandless

    Article

    Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

    Insert

    FKBP DD (L106P)-UbK0G75S
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mutation

    change all lysin residues of ubiquitin to Arg, G7…

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid

    #157933

    Purpose

    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

    Insert

    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    

    Use

    Tags

    sfGFP & TwinStrep

    Expression

    Mammalian

    Mutation

    E190D

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Pinducer20-BSD-scFv-sfGFP-DNMT3A1

    Plasmid

    #115780

    Purpose

    targeted DNA methylation tool

    Depositor

    Margaret Goodell

    Article

    Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

    Insert

    scFv-sfGFP-DNMT3A1 (DNMT3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCD5-H3CH13-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158740

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H3/CH/13 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/MI/45/15 H1N1
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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