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mCherry-VP40

Plasmid

#74421

Purpose

Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

Depositor

Judith White

Publication

Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VP40

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APEX2

Use

Gateway multisite middle entry clone

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCAP-BAC

Plasmid

#120229

Purpose

TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

Depositor

Bradley Moore

Publication

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Expression

Bacterial

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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5` CC: Puro – loxP – GFP-C

Plasmid

#219563

Purpose

5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

Depositor

Andrea Ventura

Publication

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

hPGK-promoter_PuroR_2A_loxP_GFP-C

Use

Unspecified

Promoter

hPGK

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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meffBlue chromoprotein

Plasmid

#117845

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffBlue chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffBlue

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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asPink chromoprotein

Plasmid

#117838

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, asPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-MXT GIRK2

Plasmid

#20675

Depositor

Henry Lester

Publication

Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

Insert

Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)

Use

Xenopus expression

Tags

3' UTR Xenopus B- globin and 5' UTR Xen…

Mutation

A369V

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Publication

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPEG

Plasmid

#162466

Purpose

Entry vector for gRNA insertion in plant prime editing expression vector

Depositor

Adam Bogdanove

Publication

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Type

Empty backbone

Use

Entry vector for grna

Promoter

CmYLCV

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

Plasmid

#219565

Purpose

3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

Depositor

Andrea Ventura

Publication

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

HygroR_2A_H2B-GFP-N_loxP_mScarlet

Use

Unspecified

Promoter

EF-1a

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTK204

Plasmid

#13534

Depositor

Wolf Frommer

Publication

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Bacterial

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAC-SARS-CoV-2 NSP3

Plasmid

#158354

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP3 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Publication

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GST-Keima-cb5

Plasmid

#137755

Purpose

Targets Keima to the endoplasmic reticulum. Used to monitor ER-phagy

Depositor

Carol Mercer

Publication

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Keima-cb5

Tags

GST

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FLAG-mCep290

Plasmid

#27381

Depositor

Joseph Gleeson

Publication

Valente et al Nat Genet. 2006 Jun . 38(6):623-5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cep290 (Cep290 Mouse)

Tags

FLAG

Expression

Mammalian

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Delta Spike

Plasmid

#185593

Purpose

Expresses codon-optimized full length SARS-CoV-2 Delta Spike

Depositor

Marceline Côté

Publication

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 Delta Spike (S )

Expression

Mammalian

Mutation

Contains the following mutations: T19R, Δ156-157,…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-mitfa2.1

Plasmid

#81234

Purpose

zebrafish mitfa promoter entry vector for multisite Gateway cloning

Depositor

James Lister

Insert

mitfa promoter (mitfa Zebrafish)

Use

Multisite gateway entry vector

Promoter

mitfa, 2.1kb including 5' UTR

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p2xFlag CMV2-YAP2-2nd WW mutant

Plasmid

#19047

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p2xFlag CMV2-YAP2-1st WW mutant

Plasmid

#19046

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yes-kinase associated protein isoform 3 (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine,and Proline 202…

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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piggyBAC-H2B FR-MQV

Plasmid

#194079

Purpose

Expresses nuclear-localized FR-MQV in mammalian cells

Depositor

Ralph Jimenez

Publication

Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

Insert

FR-MQV

Tags

Histone H2B

Expression

Mammalian

Mutation

Methionine 42 to Glutamine, Cystiene 159 to Valin…

Promoter

CMV7

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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