We narrowed to 26,456 results for: CAL

1. pDonor-tBFP-NLS-Neo

   Plasmid

   #80766

   Purpose

   Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   TagBFP2-3×NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #219782

   Purpose

   CaST-IRES for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCS2-BiNLS-fYap

   Plasmid

   #176623

   Purpose

   expresses optofYap in zebrafish

   Depositor

   Timothy Saunders

   Article

   Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

   Insert

   Yes associated protein (yap1 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-hSyn-Axon-jGCaMP8f

   Plasmid

   #192615

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8f.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSetB-KanR-LiLac

   Plasmid

   #184569

   Purpose

   Fluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expression

   Depositor

   Gary Yellen

   Article

   Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

   Insert

   LiLac fluorescent sensor of lactate
   

   Use

   Tags

   His7

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti Lifeact-mRuby2 BlastR

   Plasmid

   #84384

   Purpose

   Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3-myc3-ROC1

   Plasmid

   #20717

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   RING finger protein 1 (ROC1) (RBX1 Human)
   

   Use

   Tags

   3xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-OTUB1 C91S-pcDNA3.1

   Plasmid

   #118210

   Purpose

   Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLVX-TRE3G-LbNOX-Flag

   Plasmid

   #234980

   Purpose

   Expresses LbNOX in mouse cell lines

   Depositor

   Denis Titov

   Article

   Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

   Insert

   LbNOX
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLVX-TRE3G-mitoLbNOX-Flag

    Plasmid

    #234981

    Purpose

    Expresses mitochondrial LbNOX in mouse cell lines

    Depositor

    Denis Titov

    Article

    Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

    Insert

    mito-LbNOX
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LZF97_hSyn-spine-jRGECO1a

    Plasmid

    #119198

    Purpose

    Targets jRGECO1a to spines in living neurons.

    Depositor

    Don Arnold

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Rat, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin 1

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-TS2126 RnlA-Strep

    Plasmid

    #159350

    Purpose

    Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

    Depositor

    Takashi Ito

    Article

    Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

    Insert

    TS2126 RNA ligase
    

    Use

    Tags

    His6 and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-EF1Alpha-Tet-on 3G

    Plasmid

    #118592

    Purpose

    Expresses Tet-on 3G from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Tet-on 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ArcLight-Q239

    Plasmid

    #36856

    Depositor

    Vincent Pieribone

    Article

    Jin et al Neuron. 2012 Sep 6;75(5):779-85.

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    simian CMV

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-EF1A-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #125691

    Purpose

    Global labeling of excitatory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. piggyFlex

    Plasmid

    #218234

    Purpose

    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

    Depositor

    Jay Shendure

    Article

    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FKBP-myc

    Plasmid

    #20211

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FKBP (FKBP1A Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

    Plasmid

    #199349

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBR15.2-2H12.D11opt

    Plasmid

    #221185

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2.1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-N1-ACTR3

    Plasmid

    #8462

    Depositor

    Matthew Welch

    Article

    Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

    Insert

    ACTR3 (ACTR3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1-Flag HBx

    Plasmid

    #42596

    Depositor

    Ding Xue

    Article

    Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

    Insert

    Hepatitis B virus protein X
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKD-SepRS-EFSep

    Plasmid

    #34623

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Inserts

    SepRS (MMP0688 Methanocaldococcuc maripaludis)
    EF-Sep (tufB E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

    Promoter

    Ptac and Ptac (from SepRS- operon like)

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138129

    Purpose

    Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Rat, Aequorea Victoria)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCherry N1 TOMM22

    Plasmid

    #157762

    Purpose

    Expression of TOMM22:mcherry in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    TOMM22 (TOMM22 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHH0103_TEC-1/1

    Plasmid

    #91252

    Purpose

    Protein expression and purification of human SH3 domain construct TEC-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    TEC-1/1 (TEC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-SYP1-miniSOG-citrine

    Plasmid

    #50971

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    Synaptophysin-miniSOG-citrine (Syp Rat)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Expression

    Mutation

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDULE-ABK

    Plasmid

    #49086

    Purpose

    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

    Depositor

    Peter Schultz

    Article

    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

    Inserts

    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L274M, C313A, Y349F

    Promoter

    GlnRS and lpp

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3/hArf5(WT)-HA

    Plasmid

    #79427

    Purpose

    Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

    Plasmid

    #199363

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    ALFA-P2A-TagBFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAEMXT-eLACCO1.1

    Plasmid

    #167946

    Purpose

    Fluorescent biosensor for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    CD59-eLACCO1.1-GPI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

    Plasmid

    #169533

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Opto-beta2AR

    Plasmid

    #106063

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-beta2AR
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SL016: AAV-CAG-FLEX-CheRiff-GFP

    Plasmid

    #124776

    Purpose

    For AAV based expression of CheRiff in Cre-Positive cells

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    CheRiff-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

40. pAD/CMV/V5-DEST-Cdc42-2G

    Plasmid

    #68812

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Adenoviral

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge