159,352 results

1. pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)

   Plasmid

   #231733

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Inserts

   CTR9 (CTR9 Human)
   CDC73 (CDC73 Human)
   PAF1 (PAF1 Human)
   LEO1 (LEO1 Human)
   WDR61 (SKIC8 Human)
   

   Use

   Tags

   3C-Strep(II) and Flag-3C

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-DD-T6B-MUT-YFP

   Plasmid

   #235144

   Purpose

   Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

   Depositor

   Giordano Lippi

   Article

   Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

   Insert

   DHFR-fused mutant T6B peptide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   5 tryptophan residues of T6B-WT mutated

   Promoter

   hSyn

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIP4_3KT9

   Plasmid

   #234192

   Purpose

   Heterologous protein expression of aprataxin in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   3KT9 (APTX Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AiP2107 - pAAV-AiE2664m-minBG-SYFP2-WPRE3-BGHpA

   Plasmid

   #230553

   Purpose

   AiE2664m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   Bosiljka Tasic, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CA-FLEX-dtA

   Plasmid

   #232236

   Purpose

   Expresses diphtheria toxin A subunit in Cre-positive cells.

   Depositor

   Naoshige Uchida

   Article

   Tsutsui-Kimura et al bioRxiv 2022.02.05.479267

   Insert

   Diphtheria toxin A subunit
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CA

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a-Flex-MCS-WPRE-SV40 - GCH14

   Plasmid

   #230821

   Purpose

   AAV cloning vector for CRE dependent expression under the EF1a promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL1403

   Plasmid

   #226635

   Purpose

   This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

   Depositor

   Andrew Camilli

   Article

   Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

   Inserts

   tfox
   qstR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

   Plasmid

   #212705

   Purpose

   targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

   Depositor

   Elzo de Wit

   Insert

   ZFP143-FKBPV-2HA-P2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-memRFP-3xPax7gRNA

   Plasmid

   #224569

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTSAR3.1t

    Plasmid

    #210254

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCerulean
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p205-5'-Switch-ON

    Plasmid

    #217884

    Purpose

    Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #218634

    Purpose

    Proximity biotinylation of msNLRP3(ΔPYD, I125M-END)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    delPYD (I125M-END)

    Promoter

    UBC

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHTN-HaloTag_DCAF1:1-1507

    Plasmid

    #210972

    Purpose

    Mammalian expression of full-length human DCAF1. N-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DCAF1:M1-E1507 (DCAF1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pHTC-HaloTag_DCAF1:1-1507

    Plasmid

    #210973

    Purpose

    Mammalian expression of full-length human DCAF1. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    DCAF1:M1-E1507 (DCAF1 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pEJS2195_Nme2-evoFERNY-i1

    Plasmid

    #201528

    Purpose

    Mammalian expression of domain inlaid Nme2-evoFERNY-i1

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-evoFERNY-i1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEJS2197_Nme2-evoFERNY-i8

    Plasmid

    #201530

    Purpose

    Mammalian expression of domain inlaid Nme2-evoFERNY-i8

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-evoFERNY-i8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEJS2217_Nme2-rAPOBEC-nt

    Plasmid

    #201523

    Purpose

    Mammalian expression of N-terminally fused Nme2-rAPOBEC-nt

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-rAPOBEC-nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS2214_Nme2-rAPOBEC-i1

    Plasmid

    #201524

    Purpose

    Mammalian expression of domain inlaid Nme2-rAPOBEC-i1

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-rAPOBEC-i1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS2216_Nme2-rAPOBEC-i8

    Plasmid

    #201526

    Purpose

    Mammalian expression of domain inlaid Nme2-rAPOBEC-i8

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-rAPOBEC-i8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge