We narrowed to 6,480 results for: poly

1. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

   Plasmid

   #217323

   Purpose

   Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

   Plasmid

   #217320

   Purpose

   Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-GFP_mmu-miR-16-5p_sponge

   Plasmid

   #132624

   Purpose

   Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-16-5p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. UTP15:PBC028-C01:C245785

   Plasmid

   #183973

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   UTP15 (UTP15 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LIPS:SDC101a-A08:C34411

   Plasmid

   #183978

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   LIPS
   

   Use

   Baculovirus expresssion

   Tags

   C-terminal 8xHis tag:EFVEHHHHHHHH and N-termina…

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOpen-ECOligA

   Plasmid

   #165564

   Purpose

   Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   E. coli DNA Ligase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pTU1-D-spsCD

   Plasmid

   #112788

   Purpose

   Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   spsCD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTU1-A-spsCU

   Plasmid

   #112787

   Purpose

   Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   spsCU
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 47 pLIB_GST

   Plasmid

   #160272

   Purpose

   N-term 3C cleavable GST tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   polyhydrin

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPGKPhiC31obpA

    Plasmid

    #13795

    Purpose

    Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS ONE. 2007 . 2():e162.

    Insert

    PhiC31o
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. 53 pLIB_MBP

    Plasmid

    #160301

    Purpose

    N-term 3C cleavable MBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    6xHis and MBP

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1_FLAG-BPTF

    Plasmid

    #102242

    Purpose

    Expression of human Flag-BPTF using baculovirus from pFastBac1

    Depositor

    Joe Landry

    Insert

    Flag-BPTF (BPTF Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available since

    Dec. 11, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 50 pLIB_STREP

    Plasmid

    #160275

    Purpose

    N-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p406CYC1

    Plasmid

    #15975

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. Stagia3

    Plasmid

    #28177

    Depositor

    Connie Cepko

    Article

    Billings et al PLoS One. 2010 . 5(10):e13739.

    Insert

    Stop TATA
    

    Use

    Chicken expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. LEGO-GM55

    Plasmid

    #217413

    Purpose

    Luciferase expression vector with the minimal GM-CSF promoter

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human -55 to +28 minimal CSF2 promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. YWHAG

    Plasmid

    #39129

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAG (YWHAG Human)
    

    Use

    Tags

    Tev His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pRAV23

    Plasmid

    #67715

    Purpose

    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA polymerase

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pOpen-BovDNTT

    Plasmid

    #165580

    Purpose

    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    Bovine DNTT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. 56 pLIB_CBP

    Plasmid

    #160305

    Purpose

    N-term 3C cleavable CBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    CBP

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge