We narrowed to 24,440 results for: lars

1. pET-6xHis-GFP(-30)

   Plasmid

   #199167

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(-30)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRZ-CAS9-mCherry

   Plasmid

   #80974

   Purpose

   Cas9, mCherry expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHCMV_MERS-CoV_S

   Plasmid

   #207293

   Purpose

   Mammalian expression of MERS coronavirus S protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   MERS-CoV_S (S MERS coronavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3113 GST-p53

   Plasmid

   #10852

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMF1707

   Plasmid

   #125584

   Purpose

   Expresses cytosolic roGFP2 (c-roGFP2) in mammalian cells

   Depositor

   Marc Fransen

   Article

   Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

   Insert

   c-roGFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lv EF1a MS2-Fkbp2x 2A Hygro

   Plasmid

   #102807

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   MS2-Fkbp2x
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJCX3-ACTG2_R257C

   Plasmid

   #228911

   Purpose

   Expresses mutant R257C smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Use

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   R257C

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJCX3-ACTG2_WT

   Plasmid

   #228910

   Purpose

   Expresses wildtype smooth muscle actin gamma-2 in human cells

   Depositor

   Roberto Dominguez

   Insert

   Actin gamma 2, smooth muscle (ACTG2 Human)
   

   Use

   Tags

   HIS-FLAG-TEV

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Rab5a-pmCherryC1

   Plasmid

   #27679

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Rab5a (Rab5a Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHCMV_CHHF_GPC

    Plasmid

    #207260

    Purpose

    Mammalian expression of Chapare hemorrhagic fever virus GPC protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNTI820

    Plasmid

    #216629

    Purpose

    cpTurboID (V5) for mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. POM43-mTFP1

    Plasmid

    #69853

    Purpose

    N-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1 minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1/Myc-His(-)-HSulf-1

    Plasmid

    #13003

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-surfaceHA-IRES-Puro

    Plasmid

    #207321

    Purpose

    Lentiviral expression of synthetic HA surface receptor in Mammalian cells

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    HAtag-Myctag-PDGFRB-TM-IRES-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha_short (EFS)

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC-hrDNA-12.0

    Plasmid

    #206320

    Purpose

    A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

    Depositor

    Brian McStay

    Article

    van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

    Insert

    Intergenic spacer of human rDNA repeat
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 NRF2

    Plasmid

    #118708

    Purpose

    entry clone for NRF2

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    NRF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PM-APEX2

    Plasmid

    #215557

    Purpose

    Stable, inducible expression in plasma membrane

    Depositor

    Nevan Krogan

    Article

    Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

    Insert

    Lyn11-EGFP-Flag tag-APEX2-NES
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJPT002-M-HaloTag-1x FLAG

    Plasmid

    #202493

    Purpose

    Express "M" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Myristoylation site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJPT003-PM-HaloTag-1x FLAG

    Plasmid

    #202494

    Purpose

    Express "PM" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Palmitoylation-Myristoylation Site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EasyFusion T2A-H2B-GFP

    Plasmid

    #112851

    Purpose

    General donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    No

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DL015:CMV::iPAK4

    Plasmid

    #177880

    Purpose

    ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

    Plasmid

    #229387

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. DL016:CMV::eGFP-iPAK4

    Plasmid

    #177881

    Purpose

    eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. eMagBF-TgRFPT

    Plasmid

    #162253

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    eMagBF-TgRFPT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EasyFusion T2A-H2B-TagBFP

    Plasmid

    #113086

    Purpose

    General donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-TagBFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPaint-2A-TurboGFP-PEST

    Plasmid

    #67180

    Purpose

    CRISPaint universal donor for gene expression quantification using destabilized TurboGFP

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    T2A-TurboGFP-PEST
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYPQAT03

    Plasmid

    #223375

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. DL017:CMV::HaloTag-iPAK4

    Plasmid

    #177882

    Purpose

    HT-iPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLv-CMV-MyoD-ER(T)

    Plasmid

    #26809

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD-ER(T) (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only