We narrowed to 22,833 results for: Sis

1. pJMP1183

   Plasmid

   #119254

   Purpose

   rfp "test" strain

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-mTagBFP2-MRTF-A

   Plasmid

   #182061

   Purpose

   Mammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mTagBFP2-MRTF-A (MRTFA Human)
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe puro Snail

   Plasmid

   #23347

   Depositor

   Bob Weinberg

   Article

   Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

   Insert

   Snail (SNAI1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGWB445

   Plasmid

   #74831

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWB518

   Plasmid

   #74860

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with 4xMyc (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJL1-sfGFP-DQNAT

   Plasmid

   #198210

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_DQNAT
   

   Tags

   6x His Tag and DQNAT glycosylation tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB550

   Plasmid

   #74880

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mito-CIBN-GFP

   Plasmid

   #89480

   Purpose

   Encodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.

   Depositor

   Xavier Trepat Guixer

   Article

   Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

   Insert

   mito-CIBN-GFP (CIB1 Mustard Weed, Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMLV-mRFP1-P2A-Puro

   Plasmid

   #160228

   Purpose

   Expresses mRFP in mammalian cells

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   mRFP1-P2A-Puro
   

   Use

   Retroviral

   Expression

   Bacterial and Mammalian

   Promoter

   LTR

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

    Plasmid

    #200123

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENO1-BFP-NAT

    Plasmid

    #50046

    Purpose

    Expression of BFP in Candida albicans

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BFP
    

    Expression

    Yeast

    Promoter

    pENO1

    Available Since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2Apuro-Stargazin-mEGFP-iLID

    Plasmid

    #178529

    Purpose

    Expresses Stargazin-mEGFP-iLID in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Stargazin-mEGFP-iLID
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

    Plasmid

    #177841

    Purpose

    Mammalian Expression of Folate Receptor

    Depositor

    Karsten Melcher

    Article

    Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

    Insert

    Folate receptor alpha (FOLR1 Human)
    

    Expression

    Mammalian

    Mutation

    all 11 C mutated to S

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TfR-pHoran4

    Plasmid

    #61547

    Purpose

    Human transferrin receptor tagged with pH sensitive orange/red fluorescent protein pHoran4

    Depositor

    David Perrais

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    Transferrin receptor (TFRC Human)
    

    Tags

    pHoran4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB413

    Plasmid

    #74807

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. UKNP2.4.1

    Plasmid

    #98212

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pARF6(Q67L)-CFP

    Plasmid

    #11387

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Expression

    Mammalian

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHJ423

    Plasmid

    #20681

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Thr 567 to Asp

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. YFP-Cdc42(V12)

    Plasmid

    #11399

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    cell division cycle 42 (CDC42 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Glycine 12 changed to Valine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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22. Nanog-2A-mCherry

    Plasmid

    #59995

    Purpose

    Inserts mCherry immediately downstream of the Nanog coding region

    Depositor

    Rudolf Jaenisch

    Article

    Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2A-mCherry

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pGL3-24p3/LCN2-Luc

    Plasmid

    #25463

    Depositor

    Sarah Gaffen

    Article

    Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

    Insert

    24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)
    

    Use

    Luciferase

    Mutation

    none

    Available Since

    June 25, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pM-mClover3

    Plasmid

    #228624

    Purpose

    Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mClover3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

26. Level 1 P1 OsActinP:Hygint:NosT

    Plasmid

    #165423

    Purpose

    GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    

    Use

    Synthetic Biology; L1 p1(r) golden gate (moclo) h…

    Promoter

    Os Actin promoter :: Hygromycin resistance gene (…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGWB541

    Plasmid

    #74875

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCIB1(deltaNLS)-pmGFP

    Plasmid

    #28240

    Purpose

    Expresses CIB1 fused to plasma membrane-localized EGFP

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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29. SUMO_MT3

    Plasmid

    #200303

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Tags

    Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pZ_P-GAP-eGFP

    Plasmid

    #126717

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-neo

    Plasmid

    #166467

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. SpCas9-CBE6a-V106W

    Plasmid

    #215824

    Purpose

    Express CBE6a-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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34. pcDNA3.1 MYC

    Plasmid

    #176045

    Purpose

    Expresses Myc in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCherry-C1-GOLGA4-GRIP

    Plasmid

    #220068

    Purpose

    overexpreeses the TGN protein GRIP with a mCherry fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

    Insert

    GOLGA4-GRIP (GOLGA4 Human)
    

    Expression

    Mammalian

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET23b-Casp7-His

    Plasmid

    #11825

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 7 (CASP7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pDONR/zeo-MACROD1

    Plasmid

    #172572

    Purpose

    For gateway cloning of N-terminal tagged MACROD1 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Gateway cloning

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDONR/zeo-MACROD2

    Plasmid

    #172573

    Purpose

    For gateway cloning of N-terminal tagged MACROD2 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD2 (MACROD2 Human)
    

    Use

    Gateway cloning

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGWB542

    Plasmid

    #74876

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eYFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge