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pCMV-DNAJC5 (L115R)-HaloTag

Plasmid

#205711

Purpose

Mammalian expression of DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLINE1mORF1-ΔORF2-scFvFc

Plasmid

#206023

Purpose

The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D197, 199N IRES GFP

Plasmid

#195701

Purpose

Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D197, 199N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW SYT9 D330, 332N IRES GFP

Plasmid

#195702

Purpose

Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

full-length mouse SYT9 with D330, 332N mutations

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RO1.7-Hsp90ab1-FLAG

Plasmid

#206355

Purpose

AAV plasmid expressing FLAG-tagged HSP90AB1 in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Hsp90ab1 (Hsp90ab1 Mouse)

Use

AAV

Tags

3xFLAG

Promoter

1.7kb human red opsin

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1S scramble

Plasmid

#202837

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 1S scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

6

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-NeoCOVGT3-d2EGFP-mCherry

Plasmid

#201450

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT3_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT1-d2EGFP-mCherry

Plasmid

#201451

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT1_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT2-d2EGFP-mCherry

Plasmid

#201452

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT2_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-Neo-COVGT4-d2EGFP-mCherry

Plasmid

#201453

Purpose

Fluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.

Depositor

Gaetano Gargiulo

Article

Jiang et al Sci Adv. 2023 Jun 23;9(25):eadf4975. doi

Insert

COVGT4_d2EGFP-mCherry

Use

Synthetic Biology

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-IGFBP2-Clover

Plasmid

#191007

Purpose

Gateway entry vector with IGFBP2 tagged with Clover

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Gateway entry vector

Tags

Clover

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 2S scramble

Plasmid

#202839

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 2S scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-POFUT2-SVA

Plasmid

#202831

Purpose

pGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

POFUT2 intronic SVA

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-HTT-SVA

Plasmid

#202833

Purpose

pGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

HTT intronic SVA

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-CHAF1B-SVA

Plasmid

#202829

Purpose

pGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

CHAF1B intronic SVA

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1A scramble control

Plasmid

#202835

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

pLVX-SVA-lncRNA shRNA 1A scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-EGFR-FRB-GluGlu neo

Plasmid

#192293

Purpose

Retroviral expression vector for NGF-EGFR-FRB-GluGlu construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-EGFR-FRB-GluGlu (EGFR Human)

Use

Retroviral

Tags

GluGlu

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe NGF-Erbb2-2xFKBP-HA hygro

Plasmid

#192291

Purpose

Retroviral expression vector for NGF-Erbb2-2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-Erbb2-2xFKBP-HA (Erbb2 Rat)

Use

Retroviral

Tags

HA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W5F

Plasmid

#198443

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W5F

Promoter

T7 promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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5ZiPro

Plasmid

#198432

Purpose

Conformation sensitive site specific Cys-labeling

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

S84*C, R95*A, C80S, C143S, S160C, C178S,

Promoter

T7 promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W61*F

Plasmid

#198442

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W61*F, R95*A,

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W5F

Plasmid

#198436

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W5F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W83F

Plasmid

#198439

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W83F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W89F

Plasmid

#198447

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W89F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W83F

Plasmid

#198446

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W83F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W69F

Plasmid

#198438

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W69F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro W89F

Plasmid

#198440

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

W89F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gZiPro W69F

Plasmid

#198445

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W69F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro

Plasmid

#198434

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge