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We narrowed to 73,945 results for: LAS

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Showing: 7861 - 7890 of 73945 results

  1. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  20. Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R-2b]

    Plasmid
    #220431
    Purpose
    Mammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N37A/10.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody. (KCNQ1 Mouse)
    Expression
    Mammalian
    Promoter
    Dual CMV
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220431-plasmid-map-sequence-id-435314

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  21. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

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  27. T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4

    Plasmid
    #193359
    Purpose
    self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR
    Depositor
    Hans Schöler
    Article
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    CFP-e2a-SOX2AV-p2a-KLF4 (KLF4, SOX2 Human, Aequorea victoria)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Mutation
    SOX2A61V is a highly cooperative point mutant of …
    Promoter
    T7
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193359-plasmid-map-sequence-id-379851

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Showing: 7861 - 7890 of 73945 results