We narrowed to 26,456 results for: CAL

1. pET_RGG-RGG-RGG

   Plasmid

   #124938

   Purpose

   Expression of RGG-RGG-RGG

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFX-mito-eqFP650

   Plasmid

   #174179

   Purpose

   Express eqFP650 with mitochondria-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   eqFP650 with mitochondria-targeting sequence
   

   Use

   Tags

   the mitochondria-targeting sequence of the cytoch…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MELOPS

   Plasmid

   #80151

   Purpose

   Genetically encoded melanosome-localized pH sensor.

   Depositor

   Santiago Di Pietro

   Article

   Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

   Insert

   OCA2 (OCA2 Human)
   

   Use

   Tags

   mNectarine in first luminal loop

   Expression

   Mammalian

   Mutation

   nonpathologic polymorphism D257A, pore mutation V…

   Promoter

   CMV

   Available since

   Sept. 20, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pRS416Gal TDP43 WT YFP

   Plasmid

   #27447

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

5. N1-mSin1.1-GFP

   Plasmid

   #72907

   Purpose

   GFP-tagged mSin1 (isoform 1)

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   mSin1.1 (MAPKAP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pfa6a PhoCl-TsCC(B)::KANMX

   Plasmid

   #185906

   Purpose

   Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   PhoCl-TsCC(B)
   

   Use

   Tags

   PhoCl fluorophore with C-terminal TsCC(B)

   Expression

   Yeast

   Mutation

   Promoter

   SP6

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMMB67EH-Spy

   Plasmid

   #90102

   Purpose

   IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

   Depositor

   Samuel Miller

   Article

   Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

   Insert

   YcgR (ycgR )
   

   Use

   Broad-host range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPRi

   Plasmid

   #199808

   Purpose

   vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

   Depositor

   Steve Salipante

   Article

   Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

   Inserts

   dCas9
   sgRNA with GFP cassette
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-eLACCO1.1

   Plasmid

   #168789

   Purpose

   Fluorescent sensor for extracellular lactate

   Depositor

   Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

   Insert

   eLACCO1.1
   

   Use

   AAV

   Tags

   ER export and GPI

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Opto-M2R

    Plasmid

    #106070

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Klc1-GFP

    Plasmid

    #186617

    Purpose

    Encodes Kinesin Light Chain 1 fused to GFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Kinesin Light Chain 1 (KLC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DYSF-Venus

    Plasmid

    #29768

    Depositor

    Steven Vogel

    Article

    Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

    Insert

    DYSF-Venus (DYSF Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

13. Human NCKX3-FlagTag (pcDNA3.1-)

    Plasmid

    #75206

    Purpose

    Mammalian expression of SLC24A3

    Depositor

    Jonathan Lytton

    Article

    Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

    Insert

    SLC24A3 (SLC24A3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-RiboL1-jGCaMP8s

    Plasmid

    #192611

    Purpose

    AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmCherry-C1_SEPT9 i1

    Plasmid

    #71622

    Purpose

    mammalian expression of human SEPT9 with an mCherry fusion

    Depositor

    Elias Spiliotis

    Article

    Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

    Plasmid

    #199359

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-SUMOEu-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mTagBFP2-Annexin-12

    Plasmid

    #55276

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    Annexin (A4) (ANXA4 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. STOP-eGFP-ROSA26TV

    Plasmid

    #11739

    Depositor

    Klaus Rajewsky

    Article

    Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

    Insert

    STOP-eGFP-ROSA26TV
    

    Use

    Cre/Lox; /stop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAX Itgb1-FLAG

    Plasmid

    #30153

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    integrin beta 1 (Itgb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MAC_C_PTH2

    Plasmid

    #111674

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    PTH2 (PTRH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p15a_Kan_MLS_Rosa26_Short_CRISPRDel

    Plasmid

    #97008

    Purpose

    Rosa26 locus targeting vector

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAcGFP1-mito

    Plasmid

    #174180

    Purpose

    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    AcGFP with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mTFP1-Vinculin-23

    Plasmid

    #55516

    Purpose

    Localization: Focal Adhesions, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

25. syntaxin3-myc-myc-His

    Plasmid

    #12372

    Depositor

    Thomas Weimbs

    Article

    Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

    Insert

    Syntaxin3 (STX3 Human)
    

    Use

    Tags

    2xMyc-His

    Expression

    Mammalian

    Mutation

    Insert is cloned into a modified vector

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFSW GAP43-jRGECO1a P2A Syp-Halo

    Plasmid

    #185565

    Purpose

    Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

    Insert

    GAP43-jRGECO1a P2A Syp-Halo
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-eDHFR-MCS

    Plasmid

    #172108

    Purpose

    Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR

    Depositor

    Shinya Tsukiji

    Article

    Ishida et al J Am Chem Soc. 2013 Aug 28;135(34):12684

    Insert

    EGFP-eDHFR-20aa (folA E. coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

    Plasmid

    #86413

    Purpose

    nuclear split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    Nop1pr-GFP11-mCherry-PUS1
    

    Use

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    NOP1

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid

    #202585

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-hSyn-Axon-jRex-GECO

    Plasmid

    #172714

    Purpose

    AAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Axon-jRex-GECO
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Opto-M3R

    Plasmid

    #106071

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M3R; Opto-M3R; H1; 724)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M3R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pT7-EGFP-C1-HseIF4A3_I

    Plasmid

    #146581

    Purpose

    Mammalian Expression of HseIF4A3

    Depositor

    Elisa Izaurralde

    Article

    Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

    Insert

    HseIF4A3 (EIF4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCIB1(deltaNLS)-pmGFP

    Plasmid

    #28240

    Purpose

    Expresses CIB1 fused to plasma membrane-localized EGFP

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFX-EGFP-Sec61β

    Plasmid

    #174466

    Purpose

    Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZac2.1-GfaABC1D-GAT3-HA-mCherry

    Plasmid

    #117722

    Purpose

    Expresses a GABA transporter GAT-3 (Slc6a11)

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Slc6a11 (Slc6a11 Mouse)
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJAK184

    Plasmid

    #167281

    Purpose

    MazF-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Pxyl

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GWF243

    Plasmid

    #133608

    Purpose

    NS3a-CAAX-(IRES)-EGFP-DNCR2-TIAM-P2a-BFP-GNCR1-LARG

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    NS3a-CAAX, EGFP-DNCR2-TIAM, BFP-GNCR1-LARG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHR-CAG_3C-Twin-Strep

    Plasmid

    #113901

    Purpose

    Mammalian protein expression

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC57 APEX

    Plasmid

    #40306

    Purpose

    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

    Depositor

    Alice Ting

    Article

    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

    Insert

    pea APEX (Synthetic)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    K14D, W41F, E112K (relative to wild-type)

    Promoter

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTK88_GFP-Anillin

    Plasmid

    #46354

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    Anillin (ANLN Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    a1854 is changed to c to disrupt XhoI site withou…

    Promoter

    pBABE

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge