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pICSL11059

Plasmid

#68263

Purpose

Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCAX-EGFP-CIP4

Plasmid

#178363

Purpose

Expresses CIP4 c-terminal labeled with EGFP in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Cdc42 Interacting Protein 4 (TRIP10 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pWPI-E2F1

Plasmid

#114296

Purpose

Constitutive expression of the E2F1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F1 (E2F1 Human)

Use

Lentiviral

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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IRES-GFP FoxG1

Plasmid

#14935

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxG1 (FOXG1 Human)

Tags

IRES-GFP

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-TVAmCherry-2A-oG

Plasmid

#102985

Purpose

AAV expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG) after CRE-mediated recombination

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

TVA-mCherry fusion protein after CRE-mediated recombination
Optimised Rabies Glycoprotein (oG) after CRE-mediated recombination

Use

AAV; Adeno associated viral vector

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSR660

Plasmid

#65216

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-8 cap

Use

AAV

Expression

Insect

Promoter

P10 and polyhedrin

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pLV V5-LAP2beta

Plasmid

#175112

Purpose

Lentiviral expression of V5-tagged mouse Tmpo

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmpo (Tmpo Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEasiLV-MCS-coICP0

Plasmid

#235545

Purpose

To express ICP0 (doxycycline inducible)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

Plasmid

#107407

Purpose

Lentiviral expression of gRNA resistant PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

sg resistant PBRM1 (PBRM1 Human)

Use

Lentiviral

Mutation

sgRNA resistant

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbleo-ErbB1

Plasmid

#197359

Purpose

Encoding human ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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NET5s pEGFP-N2 (1154)

Plasmid

#62034

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET5s (TMEM201 Human)

Tags

EGFP

Expression

Mammalian

Mutation

short form

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPBpuro-ErbB1

Plasmid

#197358

Purpose

Encoding human ErbB1.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR (EGFR Human)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-cMyc

Plasmid

#233183

Purpose

Retroviral expression of c-Myc

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

c-Myc (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-1- LentiCRISPRv2

Plasmid

#107403

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR-WT

Plasmid

#159159

Purpose

Expresses DEPTOR in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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sgPBRM1-2- LentiCRISPRv2

Plasmid

#107404

Purpose

Lentiviral expression of Cas9 and gRNA targeting PBRM1

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

gRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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shPBRM1#32-Tet-pLKO-puro

Plasmid

#107399

Purpose

Lentiviral expression of shRNA targeting PBRM

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

shRNA targeting PBRM1 (PBRM1 Human)

Use

Lentiviral

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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