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pCMV-APOBEC1-YTHD422N

Plasmid

#194432

Purpose

APOBEC1 fused to YTHD422N

Depositor

Kate Meyer

Article

Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

Insert

APOBEC1-YTHD422N (APOBEC1 Human)

Use

CRISPR

Tags

APOBEC1-YTHD422N-HA

Expression

Mammalian

Mutation

D422N mutation in the YTH domain to enhance m6A b…

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-4mtGCaMP6f-DIO

Plasmid

#179529

Purpose

Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

Depositor

Maarten Kole

Article

Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

Has Service

AAV1

Insert

4mtGCaMP6f

Use

AAV

Tags

4x Cox8 targeting sequence

Promoter

Ef1a

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-HP1alpha

Plasmid

#179703

Purpose

visualization of centromere heterochromatin

Depositor

Kenji Sugimoto

Article

Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

Insert

heterochromatin protein alpha (CBX5 Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

Plasmid

#177850

Purpose

Bacterial Expression of AMPK

Depositor

Karsten Melcher

Article

Yan et al Science. 2021 Jul 23;373(6553):413-419.

Insert

AMPK (a1b2g1)

Tags

MBP

Expression

Bacterial

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shSV40 neo

Plasmid

#158646

Purpose

Lentiviral shRNA vector targeting simian virus 40 T antigen (SV40Tag)

Depositor

Kevin Janes

Article

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

SV40Tag (SV40gp6 Simian virus 40)

Use

Lentiviral

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF-mTET1

Plasmid

#81052

Purpose

bacterial expression of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-BRPF3

Plasmid

#65383

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF3 (BRPF3 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-SLAMF6

Plasmid

#23396

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SLAMF6 (SLAMF6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDEST15-hOPTN

Plasmid

#23052

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

optineurin (OPTN Human)

Tags

GST

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pBS mPrx1 (CT#640)

Plasmid

#13961

Depositor

Cliff Tabin

Article

Logan et al Genesis. 2002 Jun . 33(2):77-80.

Insert

Prx1 promoter (Prrx1 Mouse)

Expression

Bacterial

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf170

Plasmid

#12858

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf170

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-deltaHeat

Plasmid

#25068

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains the LRR-ROC-COR-Kinase-WD40 domains; Del…

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-PRKAA1ΔC

Plasmid

#30306

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 ΔC (Prkaa1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids Pro 526 to Gln 548

Available Since

Aug. 26, 2011

Availability

Academic Institutions and Nonprofits only
MMLV-RT(dRH) for Split-PE (pEH50)

Plasmid

#190106

Purpose

Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

MMLV-RT(delta RNAseH)

Tags

bpNLS

Expression

Mammalian

Mutation

mutations from RT in PE2 and truncation of RNAse …

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b

Plasmid

#182276

Purpose

Expresses recombinant BrCas12b

Depositor

Piyush Jain

Article

Nguyen et al EBioMedicine. 2022 Mar;77:103926. doi: 1

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis, T7, Thrombin site

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB-GST-3C-CDK1

Plasmid

#177011

Purpose

Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CDK1 (CDK1 Human)

Tags

GST-3C

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1002-pAAV-mscRE16-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163486

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP1002 - pAAV-AiE2016m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A1

Plasmid

#135503

Purpose

Mammalian expression of myc-tagged HLA-A*01:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*01:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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