We narrowed to 5,822 results for: pCas

1. HSF1 KO

   Plasmid

   #200207

   Purpose

   gRNA for HSF1 KO

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   HSF1 KO (Hsf1 Mouse)
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Sox17(2A-phiC31o)

   Plasmid

   #140762

   Purpose

   Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   phiC31o
   

   Use

   Mouse Targeting

   Promoter

   Endogenous Sox17

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Sox1(2A-Cre)

   Plasmid

   #140760

   Purpose

   Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   Cre
   

   Use

   Mouse Targeting

   Promoter

   Endogenous Sox1

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. myc-hRAD18 C207F

   Plasmid

   #68829

   Purpose

   Expresses c-myc-tagged human RAD18 with C207F mutation

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 with C207F mutation (RAD18 Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pJT112_GalL_nCDA1-xBE3

   Plasmid

   #141318

   Purpose

   Expressing base editor nCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-xBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGB2alpha2 35s:hCas9:tNos (GB0639)

   Plasmid

   #68222

   Purpose

   Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   hCas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ABE8e(TadA-8e K20A R21A)

   Plasmid

   #138496

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e K20A R21A)-nSpCas9
   

   Expression

   Mammalian

   Mutation

   TadA-8e K20A R21A

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. VRER variant

   Plasmid

   #138561

   Purpose

   Expresses human codon-optimized SpCas9-VRER and blasticidin resistance: EFS promoter-VRER-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRER
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEPQD1CB0202

   Plasmid

   #185626

   Purpose

   MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   SpCas9
   

   Use

   CRISPR and Synthetic Biology

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-sgRNA_Dicer_3

    Plasmid

    #68809

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Px458-Cas9n-Trp63-Exon4-2sgRNA

    Plasmid

    #88849

    Purpose

    CRISPR KO of Trp63

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp63 (Trp63 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

12. AtS/MAR10 insulator sequence (GB3458)

    Plasmid

    #160638

    Purpose

    pEGB3a1 AtS/MAR10

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    AtS/MAR10 insulator sequence
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Promoter

    Ef1a

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330-sgRNA_Dicer_2

    Plasmid

    #68808

    Purpose

    specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

    Depositor

    Constance Ciaudo

    Article

    Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

    Insert

    sgRNA mouse Dicer1
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

    Plasmid

    #160610

    Purpose

    Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:AsCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAT-9222

    Plasmid

    #124222

    Purpose

    Cloning plasmid for EGxFP assay with self-targeting gRNA-s

    Depositor

    Ervin Welker

    Article

    Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

    Insert

    self-targeting gRNA between EGFP halves
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1 Puro shRNA INPP5E KD1

    Plasmid

    #162004

    Purpose

    INPP5E shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5E (INPP5E Canis Lupus)
    

    Use

    Lentiviral

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hs-lambda btl

    Plasmid

    #17581

    Depositor

    Denise Montell

    Article

    Lee et al Genes Dev. 1996 Nov 15. 10(22):2912-21.

    Insert

    lambda-btl (btl Fly)
    

    Expression

    Insect

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

20. Djun - sense

    Plasmid

    #38337

    Depositor

    Norbert Perrimon

    Article

    Zhang et al Proc Natl Acad Sci U S A. 1990 Aug;87(16

    Insert

    djun (Jra Fly)
    

    Expression

    Insect

    Promoter

    Hsp70

    Available Since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only