We narrowed to 5,993 results for: pCas

Showing: 5241 - 5260 of 5993 results

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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FZ-NbPDS

Plasmid

#184268

Purpose

pAIDE containing a full-length CPSMV RNA2 cDNA modified to accomodate an NbPDS fragment.

Depositor

Feng Qu

Article

Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do

Insert

CPSMV RNA2 cDNA, modified to permit foreign gene insertion between MP and L-CP

Expression

Plant

Mutation

The protease processing site between MP and L-CP …

Promoter

CaMV 35S with duplicated enhancer

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE Cloning template vector

Plasmid

#131471

Purpose

Cloning template form ORANGE method based knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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HypaCas9

Plasmid

#138557

Purpose

Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

HypaCas9

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N692A, M694A, Q695A, H698A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1a

Plasmid

#163681

Purpose

Far-red fluorescent calcium indicator FR-GECO1a

Depositor

Robert Campbell

Article

Dalangin et al Nat Commun. 2025 Apr 7;16(1):3318. doi:

Insert

FR-GECO1a

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1c

Plasmid

#163682

Purpose

Far-red fluorescent calcium indicator FR-GECO1c

Depositor

Robert Campbell

Article

Dalangin et al Nat Commun. 2025 Apr 7;16(1):3318. doi:

Insert

FR-GECO1c

Expression

Mammalian

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHUE411

Plasmid

#62203

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

OsU3 and Ubi

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSA071 Cas9-BFP

Plasmid

#249132

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TUBA1B

Plasmid

#207763

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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px330_Rosa_sgRNA

Plasmid

#97007

Purpose

Expresses Cas9 and Rosa26 locus specific sgRNA

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Insert

Rosa26 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-Hsp70-Cas9

Plasmid

#46294

Purpose

A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

codon optimized Cas9

Use

CRISPR

Tags

3x Flag

Expression

Insect

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Hsp70

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti_PEmax-2A-MLH1dn-2A-BSD

Plasmid

#234072

Purpose

For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PEmax-2A-MLH1dn

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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