We narrowed to 9,265 results for: yeast expression

1. pnEA-NpM-SceIF4G_448-465-GB1_AF

   Plasmid

   #148715

   Purpose

   Bacterial Expression of SceIF4G_448-465

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

   Insert

   SceIF4G_448-465 (TIF4631 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV105-PPAB1-GFP-Erk1R84S-1SIM

   Plasmid

   #190253

   Purpose

   Expression Erk1-GFP-1SIM in yeast cells

   Depositor

   Liam Holt

   Article

   Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

   Insert

   ERK1R84S-SIM
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   ERK1R84S

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.027

   Plasmid

   #184964

   Purpose

   Express -Eco1 RT and ncRNA in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(wt), a1/a2 length: 12
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCL.006

   Plasmid

   #184970

   Purpose

   Express -Eco1 RT in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Integrating inducible cassette: Eco1 RT
   

   Use

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnEA-NpM-Scp20_18-49-GB1_AF

   Plasmid

   #148720

   Purpose

   Bacterial Expression of Scp20_18-49

   Depositor

   Elisa Izaurralde

   Article

   Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

   Insert

   Scp20_18-49 (CAF20 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS426-CeLACE-1

   Plasmid

   #174523

   Purpose

   Express C.elegans LACE-1 in yeast cells

   Depositor

   Oleh Khalimonchuk

   Article

   Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

   Insert

   lace-1 (C30F12.2 Nematode)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   MET25

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJo55-CA1

   Plasmid

   #159624

   Purpose

   Plasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.

   Depositor

   Vasiliy Taranov

   Article

   Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

   Insert

   C. annuum eIF4E-1
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Promoter

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSN071

   Plasmid

   #166729

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSN072

    Plasmid

    #166730

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1-NLS
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

    Plasmid

    #166842

    Purpose

    Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    G2A, C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

    Plasmid

    #166839

    Purpose

    Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    G2A, C4A, C5A, C7A

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAM1160c2

    Plasmid

    #159724

    Purpose

    Express MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Use

    Tags

    Gal4DBD and MPGIL-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAM1674

    Plasmid

    #159726

    Purpose

    Express MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Use

    Tags

    Gal4DBD and MPTLV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM1479a3

    Plasmid

    #159725

    Purpose

    Express MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCg

    Depositor

    Alexander Varshavsky

    Article

    Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

    Insert

    mDHFR (Dhfr Mouse)
    

    Use

    Tags

    Gal4DBD and MYITV-GGGGGG

    Expression

    Yeast

    Mutation

    remove start and stop codon of Dhfr

    Promoter

    ADH1

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIplac204-pOst1-ESCargo(Orange)

    Plasmid

    #140157

    Purpose

    Expresses a regulatable secretory cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. YIplac128-pOst1-ESCargo(Orange)

    Plasmid

    #140158

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIplac128-pOst1-ESCargo(Crimson)

    Plasmid

    #140156

    Purpose

    Expresses a regulatable secretory cargo for yeast at the LEU2 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPI1

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-kanMX6-P81nmt1-mEGFP

    Plasmid

    #105143

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

    Insert

    kanMX6-P81nmt1-mEGFP
    

    Use

    Tags

    81nmt1-mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    81nmt1

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMDC84-ScSEIPIN-Ndelta

    Plasmid

    #96985

    Purpose

    Express yeast SEIPIN gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    ScSEIPIN (SEI1 Budding Yeast)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    N-terminal 39 nucleotides were deleted from yeast…

    Promoter

    35S

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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