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pcDNA3.1-LSSmGFP

Plasmid

#186543

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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AttB _EGFP

Plasmid

#171597

Purpose

Strong EGFP fluorescence from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Insert

EGFP

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLX313 MSLN

Plasmid

#193617

Purpose

Expresses MSLN

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

MSLN (MSLN Human)

Use

Lentiviral

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-GCaMP6s

Plasmid

#171156

Purpose

Encodes GCaMP6s

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

GCaMP6s

Expression

Mammalian

Available Since

Aug. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-Broccoli

Plasmid

#124360

Purpose

Overexpression of circular RNA (Broccoli)

Depositor

Samie Jaffrey

Article

Litke et al Nat Biotechnol. 2019 Apr 8. pii: 10.1038

Insert

Tornado-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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mPiezo1-IRES-eGFP

Plasmid

#80925

Purpose

expresses mouse Piezo1 with GFP

Depositor

Ardem Patapoutian

Article

Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

Insert

mouse Piezo1 CDS (Piezo1 Mouse)

Expression

Mammalian

Available Since

Nov. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA

Plasmid

#225079

Purpose

Expresses precursor peptide ChrA in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_NSlmb-vhhGFP4

Plasmid

#35579

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Mammalian

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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DT0

Plasmid

#80418

Purpose

Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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