165,528 results

1. pcDNA6-N-Flag-SREBP1

   Plasmid

   #134283

   Purpose

   Expresses Flag-tagged SREBP1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

   Insert

   SREBP1 (Srebf1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mouse SREBP1 isoform a precursor

   Promoter

   CMV

   Available Since

   March 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cas9-2A-Cre

   Plasmid

   #68468

   Purpose

   co-expressing Cas9 and Cre recombinase

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Insert

   Cas9 and Cre recombinase
   

   Use

   CRISPR

   Tags

   P2A-Cre-Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeonGreen-Sec61β

   Plasmid

   #169031

   Purpose

   General ER marker

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Sec61β (SEC61B Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CaMKII-pAce-Kv2.1PR

   Plasmid

   #195529

   Purpose

   Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CaMKII

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAPB02

   Plasmid

   #65628

   Purpose

   A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

   Depositor

   Pei-yuan Qian

   Article

   Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF-G1TFAKRS

   Plasmid

   #177311

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Orton et al J Am Chem Soc. 2021 Nov 15. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

   Plasmid

   #140001

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9-NG with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-TetOne-Puro-hAXL

   Plasmid

   #124797

   Purpose

   Tet-inducible AXL expression in mammalian cells

   Depositor

   Kenneth Pienta

   Article

   Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

   Insert

   AXL (AXL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   June 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJiaDEST-APEX2-GAL3

    Plasmid

    #149518

    Purpose

    Express APEX2-Galectin-3 in mammalian cells

    Depositor

    Vojo Deretic

    Article

    Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

    Insert

    LGALS3 (LGALS3 Human)
    

    Tags

    APEX2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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