Addgene

We narrowed to 23,052 results for: Nov

Showing: 5261 - 5280 of 23052 results

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pGEX-PGC1(1-180)

Plasmid

#1028

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

contains only aa1-180 (the EcoRI site)

Promoter

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

Plasmid

#149590

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

myc eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Sox1(2A-Cre)

Plasmid

#140760

Purpose

Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Cre

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Endogenous Sox1

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 1.1kb promoter

Plasmid

#20125

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
siRNA-resistant ABHD16A-mCherry

Plasmid

#195161

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with mCherry

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-SNAP

Plasmid

#195164

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with SNAP tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

SNAP tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-Halo

Plasmid

#195163

Purpose

mammalian expression of siRNA-resistant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Rab1a N124I

Plasmid

#46778

Purpose

expression of N124I Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (Rab1a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N124I cannot stably bind guanine nucleotide

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

pSUP35, min pCYC1

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pSP65

Plasmid

#21014

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLN431 (FuGW-G8p-mKate2-1Pe )

Plasmid

#105186

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-mKate2-1Pe

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJH2069

Plasmid

#179224

Purpose

Pnmr-1::UNC-9-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-9-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-9 (unc-9 Nematode)

Use

Tags

wCherry

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1H01

Plasmid

#71090

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

nej (nej Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
pSIN-Arkadia-gRNA1

Plasmid

#180374

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-SAMTOR

Plasmid

#100517

Purpose

Expresses Myc tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)

Plasmid

#87203

Purpose

To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 108-130 are inserted.

Promoter

CMV

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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MEIS1A-pET

Plasmid

#21015

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1A (Meis1 Mouse)

Use

Tags

HIS and T7

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only