We narrowed to 10,393 results for: plasmids 101

1. pFW1000

   Plasmid

   #224210

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

   Plasmid

   #91718

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2
   

   Use

   AAV

   Promoter

   CaMKII 1.3

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TL028_AAV_hSyn:HaloTag-GluA1

   Plasmid

   #192520

   Purpose

   Expression of AMPA GluA1 with N terminal HaloTag fusion

   Depositor

   Adam Cohen

   Article

   Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

   Insert

   HaloTag-GluA1
   

   Use

   AAV and Cre/Lox

   Tags

   HaloTag

   Promoter

   hSyn

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEXfrt-EGFP-KASH

   Plasmid

   #154374

   Purpose

   Vector for Flp-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

   Plasmid

   #164514

   Purpose

   Green glucose sensor with mRuby2 fusion, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR-mRuby2
   

   Use

   AAV

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHiUGE GS-gRNA (no Cre)

   Plasmid

   #200370

   Purpose

   Backbone plasmid for cloning HiUGE GS-gRNA (without Cre)

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Type

   Empty backbone

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-TVAmCherry-2A-oG

   Plasmid

   #104330

   Purpose

   AAV constitutively expressing TVA-mCherry fusion and optimised Rabies Glycoprotein (oG)

   Depositor

   Marco Tripodi

   Article

   Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

   Inserts

   TVA-mCherry fusion protein
   Optimised Rabies Glycoprotein (oG)
   

   Use

   AAV; Adeno associated viral vector

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

   Plasmid

   #85424

   Purpose

   Lentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   anti-Her2_4D5-7_synNotch
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-fDIO-eGFP-WPRE

   Plasmid

   #203843

   Purpose

   Flp-dependent EGFP expression

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   EGFP
   

   Use

   AAV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. plYJP022-int7

    Plasmid

    #202287

    Purpose

    Plasmid containing integrase for LPattB7 site

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    int7
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL

    Plasmid

    #218761

    Purpose

    Provides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRed

    Depositor

    Lindsay Schwarz

    Article

    Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed

    Promoter

    hSyn

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC_mClover3_BSDminus_F2

    Plasmid

    #184167

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNDGG057

    Plasmid

    #231327

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pNDGG005, pOGG012, and pOGG014, NmR/KmR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry

    Plasmid

    #85436

    Purpose

    Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Pembrolizumab and anti-CTLA4 scFv
    

    Use

    Lentiviral

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pYPQ141D2.0

    Plasmid

    #99906

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMJA179

    Plasmid

    #128537

    Purpose

    Expresses anti-Prunus dulcis scFv Ab

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6
    

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLENTI-CMV-GFP-TREX1 BLAST

    Plasmid

    #164228

    Purpose

    lentiviral plasmid for GFP-hTREX1

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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