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pTRIPZ-2xFLAG-2xSTREP-E2F3A

Plasmid

#236435

Purpose

doxycycline inducible expression of E2F3A in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3A (E2f3 Mouse)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG(N)-CDK9

Plasmid

#231719

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CDK9 (CDK9 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-KNC

Plasmid

#234801

Purpose

Mammalian expression of bovine arrestin-3 (K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A, C252A, P253A, D260A, Q262A)

Depositor

Vsevolod Gurevich

Article

Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A,…

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHAND1-AM-Tag-PGK-Neo

Plasmid

#223216

Purpose

Targeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Cre/Lox; Human targeting

Tags

AM-Tag

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP-RB1sg

Plasmid

#229857

Purpose

single guide RNA targeting human RB1 gene

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

RB1sgRNA (RB1 Human)

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-AHPH

Plasmid

#187278

Purpose

Express mCherry-AHPH (RhoA biosensor)

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin and Pleckstrin homology domain (ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AHPH

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry in pmCherryN1

Plasmid

#187283

Purpose

Express IL2-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit (IL2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drN-hsAgo2

Plasmid

#221724

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for zebrafish Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drN-hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for zebrafish Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsN-drAgo2

Plasmid

#221717

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsN-drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Swap of N-domain for human Ago2 N-domain

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_sgALDH7A1

Plasmid

#218273

Purpose

CRISPR KO plasmid for ALDH7A1 in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targeting ALDH7A1 (ALDH7A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac-mProser1-FLAG-IRES-Blast

Plasmid

#226173

Purpose

Expresses full length mouse PROSER1 with 2X C-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Tags

2X FLAG

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2EF-DY

Plasmid

#221723

Purpose

Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2EF-DY (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D and F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-PRDM4-Brd 356

Plasmid

#219282

Purpose

Transcription factor PRDM4 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 21;17(1):101457. doi

Insert

PRDM4 (PRDM4 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-KIF5C

Plasmid

#221293

Purpose

Expression of KIF5C (pLxIS) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

KIF5C (pLxIS) (Kif5c Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGM84

Plasmid

#220172

Purpose

CRISRPi guide RNA against BTF3

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

BTF3 (sgRNA) (BTF3 Human)

Use

Lentiviral

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF1 CTF-FLAG

Plasmid

#217694

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF1 (ADGRF1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-566

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-tagged Sox17FNV

Plasmid

#206391

Purpose

Expresses Halo-tagged mouse SOX17FNV in mammalian cells, for single-molecule tracking.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Sox17FNV (Sox17 Mouse)

Tags

HaloTag

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVTHM-mBrn2-3xflag

Plasmid

#206390

Purpose

Expresses 3xflag fused mouse BRN2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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cacna2d1 HA D491A pcDNA3

Plasmid

#206070

Purpose

expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

Depositor

Annette Dolphin

Article

Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

D491A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-KATNA1 R14A

Plasmid

#160052

Purpose

Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNA1 (KATNA1 Human)

Tags

One-Strep-Flag (OSF)

Expression

Mammalian

Mutation

R14A mutation

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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