We narrowed to 11,686 results for: nts

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pGEX2T-TUBG1

Plasmid

#171967

Purpose

pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG1 (TUBG1 Human)

Tags

GST

Expression

Bacterial

Mutation

R275H (Please see depositor comments)

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB+BACK)

Plasmid

#169723

Purpose

Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K, Y353E

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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cpGFP-Adhiron_flex

Plasmid

#165044

Purpose

Flexible linker control for POLArISact expression in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

cpGFP-Adhiron (connected with a flexible linker)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site4 (MSP3511)

Plasmid

#160139

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-GPT1-3xFLAG

Plasmid

#163448

Purpose

lentiviral overexpression vector for human GPT1

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT1 (GPT Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLX-B3omega2 (GB1695)

Plasmid

#160644

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-B2alpha2 (GB1696)

Plasmid

#160620

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 ACMV DNA-B 1.5mer

Plasmid

#159135

Purpose

Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

Depositor

Vincent Fondong

Article

Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

Insert

ACMV DNA-B 1.5mer

Use

Infectious clone for bombarding plants

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSS02:cyt-Peredox-mCherry

Plasmid

#161743

Purpose

cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

Depositor

Markus Schwarzländer

Article

Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

Insert

cyt-Peredox-mCherry

Expression

Plant

Promoter

Ubiquitin 10

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pICH67131

Plasmid

#153223

Purpose

Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Nos promoter, NPTII, Nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FB030

Plasmid

#119714

Purpose

Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Terminator paf

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL11023

Plasmid

#117542

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S_OMEGA(pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-H2A2B

Plasmid

#106419

Purpose

H-RAN-H2A2B (RANbody H2A2B::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-H2A2B

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-RFP6

Plasmid

#106426

Purpose

H-RAN-RFP6 (RANbody RFP6::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-RFP6

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMK110

Plasmid

#99535

Purpose

contains bgl operon sequence for E. coli in vitro transcription template

Depositor

Robert Landick

Article

Kotlajich et al Elife. 2015 Jan 16;4. doi: 10.7554/eLife

Insert

E. coli bgl antisense region containing bglG

Tags

None

Promoter

lambda Pr promoter used for in vitro transcriptio…

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R465H

Plasmid

#81571

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

R465H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_2

Plasmid

#68808

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-1 (GB1204)

Plasmid

#75405

Purpose

Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

AtU6-1 promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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