We narrowed to 23,593 results for: promoter

1. pISCba-reRNA

   Plasmid

   #226823

   Purpose

   Expressing ISCba controled by PlacM promoter, guide RNA controled byPJ23119 promoter

   Depositor

   Sheng Yang

   Article

   Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

   Insert

   ISCba and its corresponding guide RNA
   

   Expression

   Bacterial

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pISClsp3-reRNA

   Plasmid

   #226824

   Purpose

   Expressing ISClsp3 controled by PlacM promoter, guide RNA controled by PJ23119 promoter

   Depositor

   Sheng Yang

   Article

   Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

   Insert

   ISClsp3 and its corresponding guide RNA
   

   Expression

   Bacterial

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-3xGFP11-mem-3xPax7gRNA

   Plasmid

   #224571

   Purpose

   Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   3xGFP11
   

   Use

   CRISPR

   Tags

   Membrane Localization Signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-hU6-2O-crBFP_EF1a-BFP

   Plasmid

   #224783

   Purpose

   BFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-hU6-2O-crEGFP_EF1a-BFP

   Plasmid

   #224784

   Purpose

   EGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crEGFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC-hU6-crBFP_EF1a-BFP

   Plasmid

   #224785

   Purpose

   BFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC-hU6-CrEGFP_EF1a-BFP

   Plasmid

   #224786

   Purpose

   EGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWR23

   Plasmid

   #202770

   Purpose

   episomal bioluminescent reporter plasmid for S. stipitis

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
   coKanMX
   ACT1 terminator (S. stipitis)
   TDH3 promoter (S. stipitis)
   coCBG
   ADH2 terminator (S. stipitis)
   ARS from S. stipitis chromosome 1
   

   Expression

   Yeast

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWR10

    Plasmid

    #202710

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWR9

    Plasmid

    #202645

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Expression

    Yeast

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid

    #216379

    Purpose

    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis and GP64 signal sequence

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

    Plasmid

    #179717

    Purpose

    Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET SUMO-FL-syt7

    Plasmid

    #213630

    Purpose

    Bacterial expression of SUMO-tagged mouse FL-syt7

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt7 (Syt7 Mouse)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR/eCas9-GPIDAF

    Plasmid

    #213710

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR/eCas9-GPIBY55

    Plasmid

    #213709

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.

    Depositor

    Qilai Huang

    Article

    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    3XFLAG and Twin-strep-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-PTK6-YF

    Plasmid

    #210715

    Purpose

    Construct for stable expression of constitutively active human PTK6 in mammalian cells.

    Depositor

    Angela Tyner

    Article

    Zheng et al Mol Cell Biol. 2010 Sep;30(17):4280-92.

    Insert

    Protein tyrosine kinase 6 (PTK6 Human)
    

    Expression

    Mammalian

    Mutation

    Tyrosine 447 changed to Phenylalanine

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. XPO6-2Strep

    Plasmid

    #210356

    Purpose

    Expresses XPO6 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    XPO6 (XPO6 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRAM2-2Strep

    Plasmid

    #210374

    Purpose

    Expresses TRAM2 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TRAM2 (TRAM2 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TRAM1-2Strep

    Plasmid

    #210352

    Purpose

    Expresses TRAM1 fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TRAM1 (TRAM1 Human)
    

    Tags

    2Strep

    Expression

    Mammalian

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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