We narrowed to 5,570 results for: SPI

1. pmCellFree_KA1_SARS-COV-2_RBD -N501Y

   Plasmid

   #169407

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   N501Y

   Promoter

   T7- CMV/CAG

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTF2I.1.0-gDNA

   Plasmid

   #132452

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   GTF2I (GTF2I Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Reb_1 aka PAS619

   Plasmid

   #71224

   Purpose

   Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   RebA G53A

   Promoter

   T7

   Available since

   April 7, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pmCellFree_KA1_SARS-COV-2_S-frag5

   Plasmid

   #169404

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   C-term frag. S protein after the furin cleavag (S SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMYU1A-TED

   Plasmid

   #154297

   Purpose

   A shuttle vector between E. coli and magnetotactic bacteria (e.g. Magnetospirillum) containing a TED module

   Depositor

   Shigehiko Yumura

   Article

   Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest490-OPN-a

   Plasmid

   #17590

   Depositor

   Xin Wang

   Article

   Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

   Insert

   OPN-a (SPP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Base pairs 49–942

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCR3.1-OPNa

   Plasmid

   #107980

   Purpose

   expression of full-length OPN

   Depositor

   Georg Weber

   Article

   He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

   Insert

   full-length osteopontin (SPP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest490-OPN-c

   Plasmid

   #17592

   Depositor

   Xin Wang

   Article

   Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

   Insert

   OPN-c (SPP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Base pairs 1–93, 175–942

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETDuet-T7-Ct-YhjH

   Plasmid

   #79167

   Purpose

   Dual expression of Ct biosensor and YhjH under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   yhjH
   T7-Spinach2-Ct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIVEX-anchor

    Plasmid

    #46844

    Purpose

    Template for the amplification of spiking anchors in BeSD

    Depositor

    Florian Hollfelder

    Article

    Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest490-OPN-b

    Plasmid

    #17591

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-b (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 1–175, 217–942; See Depositor Comments

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR3.1-OPNc

    Plasmid

    #107982

    Purpose

    expression of OPN splice variant c

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    osteopontin splice variant c (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splice variant

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet-T7-Ct-WspR

    Plasmid

    #79166

    Purpose

    Dual expression of Ct biosensor and WspR WT under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    WspR (wspR P. areuginosa)
    T7-Spinach2-Ct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCR3.1-OPNb

    Plasmid

    #107981

    Purpose

    expression of OPN splice variant b

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    osteopontin splice variant b (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    splice variant

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDest490-OPN-NT

    Plasmid

    #17594

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-NT (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 49-498

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCR3.1-OPNc PSM5

    Plasmid

    #106330

    Purpose

    Mammalian expression of mutant osteopontin

    Depositor

    Georg Weber

    Article

    Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

    Insert

    OPNc (SPP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    OPNc phosphorylation site mutant SGSSEEKQNAVSSEET…

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest490-OPN-10 kDa

    Plasmid

    #17595

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-10 kDa (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 499–630

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pDest490-OPN-CT

    Plasmid

    #17593

    Depositor

    Xin Wang

    Article

    Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

    Insert

    OPN-CT (SPP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Base pairs 631–942

    Promoter

    Available since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pEYC1A

    Plasmid

    #127758

    Purpose

    Research the effect of gene orientation of transcription

    Depositor

    Richard Murray

    Article

    Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

    Insert

    mSpinach, MG RNA aptamer in convergent orientation
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. DGP104- pSP64 Poly(A)700

    Plasmid

    #70098

    Purpose

    an RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kinetics

    Depositor

    David Gresham

    Article

    Neymotin et al RNA. 2014 Oct;20(10):1645-52. doi: 10.12

    Insert

    700bp insert from B. subtilis
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only