We narrowed to 11,735 results for: pcdna

1. pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

   Plasmid

   #177770

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T139A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

   Plasmid

   #177771

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K370A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

   Plasmid

   #177772

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S374A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

   Plasmid

   #177773

   Purpose

   Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K592A

   Promoter

   Available since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-HA-HsSmg5-G120E_W

   Plasmid

   #147819

   Purpose

   Mammalian Expression of HsSmg5-G120E

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg5-G120E (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-MS2-HA-HsSmg7_633-1091_T

   Plasmid

   #147558

   Purpose

   Mammalian Expression of HsSmg7_633-1091

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg7_633-1091 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation V854I compared to the seq…

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-myc-TRBP-dsRBD2

   Plasmid

   #180602

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-myc-TRBP-dsRBD1

   Plasmid

   #180601

   Purpose

   Expressing various parts of TRBP

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

   Insert

   TRBP (TARBP2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-α-COPI-WD40-Arg57Ala

   Plasmid

   #180759

   Purpose

   Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Arg57Ala (residues 1-327)

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

   Insert

   α-COPI-WD40-Arg57Ala
   

   Use

   Tags

   C-terminal strep tag

   Expression

   Mammalian

   Mutation

   Encodes residues 1-327; mutations: Arg57Ala, Leu1…

   Promoter

   CMV

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-α-COPI-WD40-Tyr139Ala

    Plasmid

    #180761

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Tyr139Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Tyr139Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Tyr139Ala, Leu…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-α-COPI-WD40-Asp115Ala

    Plasmid

    #180760

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40-Asp115Ala
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Asp115Ala, Leu…

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-TRBP-L2+D2+L3-myc/His

    Plasmid

    #180600

    Purpose

    Expressing various parts of TRBP

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al Nucleic Acids Res. 2018 Sep 28;46(17):91

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Flag-gp130dimer-stm2585

    Plasmid

    #174395

    Purpose

    Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    IL6ST cytosolic domain and sarA chimera
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-Flag-stm2585-gp130

    Plasmid

    #174393

    Purpose

    Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    140-177 of sarA replaced with homologous IL6ST se…

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3HA-AMOT p130∆PPxY2

    Plasmid

    #166712

    Purpose

    expresses AMOT with inactivating mutation in second PPxY motif in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 P262A P263A (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed proline 262 to alanine, proline 263 to al…

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3HA-AMOT p130∆PPxY3

    Plasmid

    #166713

    Purpose

    expresses AMOT with inactivating mutation in third PPxY motif in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 P3072A P308A (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed proline 307 to alanine, proline 308 to al…

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-mas-PRGRH-Nluc

    Plasmid

    #158608

    Purpose

    Plasmid expressing PRG(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - PDZ RhoGEF RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-mas-p115RH-Nluc

    Plasmid

    #158607

    Purpose

    Plasmid expressing p115(RH)-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - p115RhoGEF RH domain - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3_PKA-CαN (E12,14A)-mVenus

    Plasmid

    #168499

    Purpose

    Mammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3_PKA-CαN (K8,9R)-mVenus

    Plasmid

    #168498

    Purpose

    Mammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge