We narrowed to 12,047 results for: LEA

Showing: 5301 - 5320 of 12047 results

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pIRES_iSeroSnFR_cytosolic

Plasmid

#214132

Purpose

Fluorescent biosensor for intracellular serotonin (5-HT)

Depositor

Steffen Sinning

Article

Bukowski et al Sci Rep. 2024 Mar 19;14(1):6541. doi: 10

Insert

iSeroSnFR_cytosolic

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRegev_ExonSkip_LandingPad

Plasmid

#204186

Purpose

Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

Beta globin splicing minigene

Use

Synthetic Biology

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-LbCas12a-Plus-mCherry

Plasmid

#191230

Purpose

To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

Depositor

Zhili Rong

Article

Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

Insert

LbCas12a-Plus-P2A-mCherry

Use

CRISPR

Mutation

D156R, R883K, R887A

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMAH-ER-bapaUGAc

Plasmid

#201674

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in ER

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

ER-bapaUGAc

Tags

ER leading sequence and ER retention sequence

Expression

Mammalian

Promoter

CMV SP6

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-Citrine-NLS

Plasmid

#182583

Purpose

Transient expression of Citrine-NLS in plant cell (Nucleus)

Depositor

Yutaka Kodama

Article

Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

Insert

NLS

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-FKBP-mTagBFP2-MCS

Plasmid

#178864

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-26

Plasmid

#172751

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-26

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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His-SUMO-hDDI2-D252N_pET11a

Plasmid

#169122

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Mutation

D252N

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-eDHFR-SIMvada

Plasmid

#164649

Purpose

SIM mutant tagged with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

SIMvada

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-API5

Plasmid

#157655

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

His tag

Expression

Bacterial

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4-ZfCry4

Plasmid

#136242

Purpose

Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

ZfCry4

Tags

Flag

Expression

Mammalian

Mutation

D196H- Please see depositor comments below

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI165

Plasmid

#126074

Purpose

Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
HcRed1-Nucleus-7

Plasmid

#55843

Purpose

Localization: Nucleus, Excitation: 588, Emission: 618

Depositor

Michael Davidson

Insert

SV40 T-Antigen Nuclear Localization Signal (NLS)

Tags

HcRed1-NLS

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-Kdrl-5p-FokI-EL

Plasmid

#35684

Depositor

Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-5p-FokI-EL

Use

Mrna production

Tags

FLAG and Flag

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pEMS1192

Plasmid

#29098

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple165 (PKP2 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKAsub-YPet-NES

Plasmid

#138203

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub-YPet-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-Rheb(5A)

Plasmid

#192439

Purpose

Encodes mCherry-tagged Rheb lacking residues 38-42.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb amino acids 38-42 deleted.

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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hFLT1 delta 754-763

Plasmid

#86035

Purpose

human VEGFR1 with a 10 amino acid deletion in EC close to TM domain

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 delta754-763 aa (FLT1 Human)

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

delta 754-763

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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