We narrowed to 14,011 results for: flag

1. AAK1

   Plasmid

   #73244

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   AAK1 (AAK1 Human)
   

   Use

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 47-307

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLenti spCas9 T2A iRFP670 P2A puro

   Plasmid

   #122182

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   iRFP670
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TH

   Plasmid

   #39079

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TH (TH Human)
   

   Use

   Tags

   Tev His Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pBJ-mPar3

   Plasmid

   #53230

   Purpose

   mammalian expression of mouse Par3

   Depositor

   Shaun Coughlin

   Insert

   Par3 (F2rl2 Mouse)
   

   Use

   Tags

   FLAG and bovine prolactin signal sequence (MDSKGS…

   Expression

   Mammalian

   Mutation

   Deletion of aa1-15 (native signal peptide)

   Promoter

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

5. cdk1 FRET sensor (2327)

   Plasmid

   #26064

   Depositor

   Jonathon Pines

   Article

   Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

   Insert

   Cdk1 FRET Sensor
   

   Use

   Tags

   CFP variant, FLAG, and YFP variant

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-CRISPR-Puro

   Plasmid

   #208646

   Purpose

   pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Clawed frog nucleoplasmin NLS, Flag, Puromycin re…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOttc1472 - pAAV CaMKII KDELR1-Myc-DDK

   Plasmid

   #192599

   Purpose

   An AAV packaging vector that expresses KDELR1 under control of the CaMKII promoter.

   Depositor

   Brandon Harvey

   Insert

   KDELR1 (KDELR1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CaMKII

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti spCas9 T2A mNeonGreen P2A puro

   Plasmid

   #122183

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   mNeonGreen
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti spCas9 T2A TagRFP-T P2A puro

   Plasmid

   #122200

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9
   TagRFP-T
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOttc1501 - pscAAV CMV-IE hCDNF

    Plasmid

    #188538

    Purpose

    An AAV packaging vector that expresses hCDNF under control of the CMV-IE promoter.

    Depositor

    Brandon Harvey

    Insert

    hCDNF (CDNF Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV-IE

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLIPf-NK1R Control Plasmid

    Plasmid

    #101125

    Purpose

    Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells

    Depositor

    New England Biolabs, Ana Egana

    Insert

    Neurokinin-1 Receptor, Truncated (TACR1 Human)
    

    Use

    Tags

    10xHis, CLIP-tag (CLIPf), and FLAG Epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. TRMT10C (RG9MTD1A-c113)

    Plasmid

    #110248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RG9MTD1 (TRMT10C Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid

    #174902

    Purpose

    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Y306A, Y384F

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7oGFP

    Plasmid

    #69346

    Purpose

    Orthogonal GFP message under T7 promoter

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    Super folder GFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. PKD2 (PKD2A-c034)

    Plasmid

    #98226

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PKD2A (PKD2 Human)
    

    Use

    Baculovirus

    Tags

    TEV-His10-Flag

    Expression

    Mutation

    pfam00520:Ion_trans 475-680

    Promoter

    polyhedrin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pResQ shFEN3 3XF-FEN1 DeltaC

    Plasmid

    #17754

    Depositor

    Sheila Stewart

    Insert

    Flap Endonuclease I (FEN1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Triple Flag

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal 20 amino acids from FEN1

    Promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

17. DNPEP

    Plasmid

    #39040

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DNPEP (DNPEP Human)
    

    Use

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pFETCh_HHEX

    Plasmid

    #72353

    Purpose

    Donor vector for 3' FLAG tag of human HHEX

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    HHEX Homology Arms (HHEX Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. CA6

    Plasmid

    #38992

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CA6 (CA6 Human)
    

    Use

    Tags

    Tev His Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. p14-9VN9-3A

    Plasmid

    #50932

    Purpose

    Expresses human SRP14 and SRP9-3A proteins as a fusion protein in mammalian cells.

    Depositor

    Katharina Strub

    Insert

    SRP14 and SRP9 (SRP14, SRP9 Human)
    

    Use

    Tags

    VN tag and flag tag

    Expression

    Mammalian

    Mutation

    Amino acids 60, 61 and 64 of SRP9 changed to alan…

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pLenti-HA-CHAC1

    Plasmid

    #242710

    Purpose

    human CHAC1 gene expression

    Depositor

    Xingguo Zhu

    Insert

    CHAC1 (CHAC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Multiple wobble mutations

    Promoter

    CMV

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pB-CuR-hSOD1

    Plasmid

    #232477

    Purpose

    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-CuO

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLVX-FFSS-Cyclin D1

    Plasmid

    #215151

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT3-EF1aH Yap S127A

    Plasmid

    #86497

    Purpose

    Mammalian expression of FLAG-tagged Yap containing S127A mutation

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes associated protein 1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLVX-TetOne-FFSS-Cyclin D1

    Plasmid

    #215146

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLVX-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215152

    Purpose

    Expresses FFSS-Cyclin D1 in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215147

    Purpose

    Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    TRE3GS

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid

    #171801

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-hgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPHAGE-CR-NENF-C-TAP

    Plasmid

    #128511

    Purpose

    Lentiviral constitutive expression of CRISPR-resistant NENF with c-terminal FLAG and HA tag.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    NENF (NENF Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Synonymous silent mutations to F81, Y82, G83 and …

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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