We narrowed to 11,499 results for: aga

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-scrambled

Plasmid

#183455

Purpose

This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

scrambled sgRNA

Use

Lentiviral

Promoter

shRNA: H1 / gene: ubiquitin

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA1

Plasmid

#185002

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA1 (Akp3 Mouse)

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168244

Purpose

"Rescue Rac-CA expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-CA

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-sgSLC35B2

Plasmid

#83932

Purpose

Lentiviral vector expressing an sgRNA targeting SLC35B2 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgSLC35B2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdkn3

Plasmid

#160955

Purpose

Cdkn3 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdkn3 shRNA (Cdkn3 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P PRIM2_2

Plasmid

#160796

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_2

Use

Lentiviral

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P ERCC2_2

Plasmid

#160782

Purpose

Suppress ERCC2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shERCC2_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA

Plasmid

#108812

Purpose

Expresses aa324 to C-terminus of TP901 integrase with N-term FKBP and C-term NLS

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

TP901 integrase fragment aa324 to C-terminus

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

pCAG

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpA

Plasmid

#108811

Purpose

Expresses N-terminus to aa323 of TP901 integrase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

TP901 integrase fragment N-terminus to aa323

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2693_pCAG-PV1-iCre-N251-L1-nMag-NLS-BGHpA

Plasmid

#108819

Purpose

Expresses N-terminus to aa251 of Cre recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa251

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA

Plasmid

#108820

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2697_pCAG-PV1-iCre-N256-L1-nMag-NLS-BGHpA

Plasmid

#108821

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA

Plasmid

#108822

Purpose

Expresses aa257 to C-terminus of Cre recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa257 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1346_pCAG-PV1-PhiC31-N428-L1-FRB-NLS-BGHpA

Plasmid

#108793

Purpose

Expresses N-terminus to aa428 of PhiC31 integrase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment N-terminus to aa428

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW1359_pCAG-PV1-FKBP-L1-PhiC31-429C-NLS-BGHpA

Plasmid

#108794

Purpose

Expresses aa429 to C-terminus of PhiC31 integrase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa429 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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