We narrowed to 12,771 results for: NUC

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pmMBD2.4G pc2841

Plasmid

#229561

Purpose

Mammalian expression vector codes for MBD2(aa 235-414) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Bacterial

Mutation

MBD2(aa 235-414)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-6G pc2843

Plasmid

#229562

Purpose

Mammalian expression vector codes for MBD2(aa 153-235) fused c-terminal to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Bacterial

Mutation

MBD2(aa 153-235)

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD1∆CxxC3 pc4783

Plasmid

#229748

Purpose

Bacterial expression vector of MBD1 with a deletion of CXXC3 domain. C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD1 (Mbd1 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD1 lacking the third CXXC domain

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD4 pc4785

Plasmid

#229750

Purpose

Bacterial expression vector of MBD4 C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD4 (Mbd4 Mouse)

Tags

Intein

Expression

Bacterial

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD2∆N(MBD2b) pc4787

Plasmid

#229752

Purpose

Bacterial expression plasmid of MBD2∆N(MBD2b) lacking the N-terminus: aa 1-152. C-terminal tagged to intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD2 lacking the N-terminus: aa 1-152.

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M80

Plasmid

#242632

Purpose

MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

mCherry (MYC Human)

Use

RNAi

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M74

Plasmid

#242626

Purpose

MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

MYC (MYC Human)

Use

RNAi

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M56B

Plasmid

#242625

Purpose

Dox-inducible MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

MYC (MYC Human)

Use

RNAi

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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nb-enDR3-TEV-mEGFP-NLS (pRS1458)

Plasmid

#240547

Purpose

Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.

Depositor

Roman Szczęsny

Article

Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

nb-enDR3

Expression

Mammalian

Mutation

S48A, K50A

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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phSyn2 sfGFP-RNF152

Plasmid

#215374

Purpose

Lentiviral expression of sfGFP-tagged lysosome membrane protein RNF152 under the neuron-specific human synapsin promoter.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

RNF152 (RNF152 Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY_SlTH51-IP

Plasmid

#235993

Purpose

Expresses SpRY_SlTH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_ATH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 8 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY_MTH51-IP

Plasmid

#235992

Purpose

Expresses SpRY_MTH51 in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY_MTH51

Use

CRISPR

Expression

Mammalian

Mutation

ectopic insertion of 7 amino acids of PID turn-he…

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-FLAG-coK8

Plasmid

#235542

Purpose

To express tagBFP-2A-FLAG-coK8 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

K8

Use

Lentiviral

Tags

FLAG

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-coICP0-∆USP7

Plasmid

#235540

Purpose

To express tagBFP-2A-coICP0-∆USP7 (ICP0 ∆618-633, co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0-∆USP7

Use

Lentiviral

Mutation

Deletion of region ∆618-633.

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_IFNB1_gRNA_1

Plasmid

#235532

Purpose

gRNA against human IFNB1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IFNB1 (IFNB1 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_IFNB1_gRNA_3

Plasmid

#235533

Purpose

gRNA against human IFNB1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IFNB1 (IFNB1 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-ZNF84

Plasmid

#226450

Purpose

For subcloning of human ZNF84 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

ZNF84 (ZNF84 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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TOPO-CHR12:132.68MB LG4

Plasmid

#226454

Purpose

Encodes an LG4 enhancer for subcloning or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

CHR12:132.68MB LG4

Use

Cloning vector

Mutation

No

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3658_pPV2-NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Plasmid

#239699

Purpose

BsaI Golden Gate assembly plasmid encoding the COMET NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG2(WT)

Plasmid

#79432

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG2 (ARFGEF2 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(WT)

Plasmid

#79431

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

C1758R

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-PCNAL2 (pc3385)

Plasmid

#213140

Purpose

Expresses human PCNA miRFP670 tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2021 Dec 16;49(22):12

Insert

PCNA (PCNA Human)

Tags

miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-WT-sgNT

Plasmid

#222120

Purpose

EIF3D overexpression vector with non-targeting sgRNA

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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PE-pegRNA(BG)-nsgRNA(BG)

Plasmid

#232727

Purpose

A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

Depositor

David Brafman

Article

Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

Insert

pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-RNAgateDel-sgEIF3D

Plasmid

#222137

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

V291-F306 deletion

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

Plasmid

#228533

Purpose

A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pADSS-EGFP

Plasmid

#228197

Purpose

expressed human ADSS with an EGFP fluorescent protein tag

Depositor

Stephen Benkovic

Article

Zhao et al J Biol Chem. 2015 Mar 13;290(11):6705-13

Insert

ADSS (ADSS1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2386

Plasmid

#225639

Purpose

AAV plasmid with Ple388 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

Insert

Ple388 (PITX3 Human)

Use

AAV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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