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pcDNA6-rbN1 S77A

Plasmid

#32945

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

SLc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Change Serine77 to Alanine

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pSG 417

Plasmid

#63864

Purpose

Detect translation initiation from AUU start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUU start codon

Promoter

PtacI

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
FLAG-ADA add back mutant pCMV5

Plasmid

#17554

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

FLAG-FoxO1-ADA

Tags

FLAG

Expression

Mammalian

Mutation

siRNA-resistant Foxo1 by replacing 3 residues (un…

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only
pTH651-RLuc/minFLuc

Plasmid

#29697

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Available Since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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As5 (pBW414J105-arsR)

Plasmid

#123162

Purpose

Arsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J105-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J105, ParsR

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Article

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSG 416

Plasmid

#63863

Purpose

Detect translation initiation from AUA start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

AUA start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

Plasmid

#126025

Purpose

express RFP- and mCer-tagged LARIAT for optogenetic clustering

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

WT

Promoter

Hsp70

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-HAT-SP65

Plasmid

#21090

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT domains aa 1107-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
p416TEF-NQYFP

Plasmid

#99552

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Article

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 LacZ

Plasmid

#123292

Purpose

Mammalian expression of control truncated LacZ

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

LacZ

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F146A

Plasmid

#45973

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F146A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G0-R20

Plasmid

#101818

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G20-R0

Plasmid

#101807

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSALECTNK-LGK/csTEM1

Plasmid

#81164

Purpose

pSALECT plasmid with levoglucosan kinase fused to TEM-1

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

Levoglucosan kinase

Tags

Codon swapped TEM-1 beta-lactamase (H26 to W290) …

Expression

Bacterial

Promoter

lac

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-2a-1

Plasmid

#22764

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 2a-1 (mir-2a-1 primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-13a

Plasmid

#22761

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13a (mir-13a primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA6-rbN1 S77D

Plasmid

#32947

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

Insert

slc9a3r1 (SLC9A3R1 rabbit)

Tags

T7 tag, his, and partial FLAG

Expression

Mammalian

Mutation

Serine 77 mutated to Aspartic Acid

Promoter

CMV

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pFbx15(-640/-527)-TK-Luc

Plasmid

#13413

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pISH-Gucy1b2-B

Plasmid

#105457

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Bleymehl et al Neuron. 2016 Dec 21;92(6):1196-1203. doi

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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