We narrowed to 29,518 results for: REP

1. PXPR007 sgATXN1L-2

   Plasmid

   #74963

   Purpose

   Cas9 + sgATXN1L-2 with blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   ATXN1L (ATXN1L Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2_hSyn_Phobos_CA_Citrine

   Plasmid

   #98218

   Purpose

   Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos_C128A gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

   Promoter

   human synapsin

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Neuroligin-2 scFv [L107/90]

   Plasmid

   #206763

   Purpose

   Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/90 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP

   Plasmid

   #50834

   Purpose

   An AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.

   Depositor

   Brandon Harvey

   Article

   McDevitt et al Cell Rep. 2014 Sep 25;8(6):1857-1869. do

   Insert

   hChR2 (H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   EF1a

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EFS-hTSC1-T2A-Bsd

   Plasmid

   #193199

   Purpose

   Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   TSC1 (TSC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-VTKD3

   Plasmid

   #170848

   Purpose

   AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-VTKD4

   Plasmid

   #170849

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-VTKD5

   Plasmid

   #170850

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-VTKD6

   Plasmid

   #170851

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNL3.1-Erg

    Plasmid

    #182706

    Purpose

    Express Erg enhancer in mammalian cells

    Depositor

    Robert Rowe

    Article

    Wang et al Cell Rep. 2022 Apr 5;39(1):110587. doi:

    Insert

    Enhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_GP1Fc Cl13

    Plasmid

    #138599

    Purpose

    Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)

    Depositor

    Annette Oxenius

    Article

    Krautler et al Cell Rep. 2020 Jan 28;30(4):997-1012.e6.

    Insert

    LCMV GP1-Fc
    

    Use

    Tags

    Fc fusion protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-PE SpG

    Plasmid

    #179081

    Purpose

    All-in-one prime editor piggyBac transposon, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNL-CEF-IgM

    Plasmid

    #187013

    Purpose

    Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

    Plasmid

    #182547

    Purpose

    Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

    Depositor

    Robert de Vries

    Article

    Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

    Insert

    H5_Vietnam_1203_04_Y161A
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Y161A mutant that confers exclusive NeuAc to NeuG…

    Promoter

    CMV

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. NCKX4 scFv [N414/25]

    Plasmid

    #190553

    Purpose

    Mammalian Expression of NCKX4 scFV. Derived from hybridoma N414/25.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    NCKX4 (Homo sapiens) recombinant scFV (Slc24a4 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-VTKS4-GCaMP6f

    Plasmid

    #178730

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-mNeonGreen-BRD4-p300

    Plasmid

    #204697

    Purpose

    This plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4-p300 (BRD4, EP300 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    direct fusion of BRD4 to p300

    Promoter

    CMV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid

    #51257

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248,789,805,817,848,9…

    Promoter

    EF1a

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only