We narrowed to 12,220 results for: cel.2

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myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
GST-RAPH1-F2

Plasmid

#194864

Purpose

Express the RAPH1 fragment (535-868) tagged to GST in bacteria.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 1062-1250 (RAPH1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1062-1250

Promoter

Tac

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_FGF21

Plasmid

#182406

Purpose

Bacterial expression of fgf21

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

fgf21 (FGF21 Human)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

Plasmid

#182393

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ
CydAB-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LLP730_L2_pV01_0I_FRT-OCS-TB-rLUC_TCTP-fLUC

Plasmid

#192373

Purpose

To test for the repressive potential of the OCS and Transcription Block (TB) fusion with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP729_L2_pV01_0I_FRT-OCS-rLUC_TCTP-fLUC

Plasmid

#192372

Purpose

To test for the repressive potential of the OCS with the FRT sites around it. Then used as a control to represent the off state of many cicuits.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP734_L2pV1_1I_OCS-rLUC_F-NOS-FlpO

Plasmid

#192377

Purpose

To test if the NOS promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO

Plasmid

#192378

Purpose

To test if the TCTP promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-OCS-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-mfn2 guide

Plasmid

#121993

Purpose

An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mfn2 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c-opa1 guide

Plasmid

#121995

Purpose

An entry vector with U6a and U6c promoter driving opa1 guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

opa1 gRNA

Use

CRISPR

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9

Plasmid

#100162

Purpose

Expresses ZFYV9

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

ZFYV9 (ZFYVE9 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4b-ISH-2kb

Plasmid

#68030

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4B (Sema4b Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4f-ISH-2kb

Plasmid

#68033

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4F (Sema4f Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Tags

2X myc

Expression

Insect

Promoter

Hsp70

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
XE156 delta LZ-XDpr-CS2+

Plasmid

#16766

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

delta LZ-XDpr (dact1-b Frog)

Use

Xenopus expression

Mutation

deletion of first 129 amino acids, including the …

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE154 XDpr-pt mutant-CS2+

Plasmid

#16768

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-pt mutant (dact1-b Frog)

Use

Xenopus expression

Mutation

last four amino acids mutated to MNTV instead of …

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

Plasmid

#159130

Purpose

expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_FBXO22

Plasmid

#159127

Purpose

expresses FBXO22 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

FBXO22 (FBXO22 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

Plasmid

#159128

Purpose

expresses KEAP1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

KEAP1 (KEAP1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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